Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 5Analyzing End-Point DataManually CallingAllele Types1. Select the Results tab.2. In the Allelic Discrimination Plot, zoom out until all crossmarks are visible inthe plot.a. Click (magnifying glass tool).b. Click the plot to zoom out.c. Click (lasso tool).d. Select all of the marks within the plot by clicking and dragging the pointeracross all datapoints in the plot.The software outlines all selected wells within the grid view.e. Examine the tray pane to confirm that all wells are selected. If not all wellsare selected, repeat steps a through d until all wells are visible on the plot.Black border(surrounding selected wells)3. Select the sample cluster exhibiting amplification of the first probe.Allele Xhomozygotes5-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_End-Point.fm