Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 6Analyzing Real-Time DataAdding PlateDocuments tothe StudyTo conduct a comparative analysis of a series of relative quantification plates in astudy, you must first add the plates to a Relative Quantification Multiple PlateDocument.Plate documents added to a study must:• Be of the same consumable format (for example, you cannot compare samplesrun on 384-well plates to those run on 96-well plates)• Have been run using the same method (identical thermal cycler conditions,sample volume, and data collection options)• Contain sample names for all In Use wells. (If a plate document does notcontain samples, you can apply sample names to it using the SDS software.)IMPORTANT! Relative Quantification Multiple Plate Documents exclude from theanalysis all wells of plates that do not have a sample name applied to them.IMPORTANT! Relative Quantification Multiple Plate Documents cannot comparedata from runs performed using different plate formats (for example, samples run ina 96-well plate cannot be compared to those run in a 384-well plate).To add plates to the Relative Quantification Multiple Plate Document:1. In the multiple plate document, click .2. In the Add dialog box, do one of the following:If you know where your plate documents are located:a. Select the File tab.b. Navigate to the directory containing the relative quantification platedocument files of interest.c. Press and hold the Ctrl key, select the files of interest, thenclick .Added platedocument filesSelected platedocument filesIf you do not know where your plate documents are located, or want to searchfor specific plate documents:a. Select the Search tab.b. In the Search in field, enter a directory address to search or click and selecta location (directory or hard drive) to search.c. If you want the SDS software to search all subdirectories in the chosenlocation, select Include Subdirectories.6-24 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm