Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 6Analyzing Real-Time DataGene ExpressionSettings for StudySettingsThe settings in this group box determine how the software handles the endogenouscontrol data and RQ Min/Max confidence values for your study. These settings arecrucial to the analysis and are set once for the study.Calibrator Sample drop-down listEndogenous Control Detectordrop-down listControl Type option buttonsRQ Min/Max Confidence drop-down listFigure 6-11Gene Expression Settings for Study Settings• Calibrator Sample – Displays the name of the sample that the software uses asthe calibrator for the study.• Endogenous Control Detector – Specifies the name of the detector that thesoftware will use as the endogenous control for the study.• Control Type – The Multiplexed/Non-Multiplexed option buttons specify howthe software will use the data from the wells labeled with the endogenouscontrol data.• RQ Min/Max Confidence – Specifies the confidence value that the softwarewill use to calculate the standard error of the mean expression level (RQMaxand RQMin values) for the samples in the study.• Export Individual ∆C T s – Instructs the software to export the ∆C T values ofindividual samples in the analysis instead of the average ∆C T values for thereplicate group.6-28 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm