Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Workflow OverviewAllelicDiscriminationWorkflow1. Create an allelic discrimination plate document (see page 3-9).2. * Apply markers to the plate document:a. Create detectors for the allelic discrimination probes (see page 3-11).b. Create a marker for each allelic discrimination probe pairing (see page 3-14).c. Copy the marker(s) to the plate document (see page 3-15).3. * Assign detector tasks to the wells of the plate document (NTC and Unknown)(see page 3-16).4. If you would like to perform thermal cycling of the allelic discrimination plate onthe 7900HT instrument, create a real-time plate document for the plate andprogram it with the method for the allelic discrimination run. Otherwise, continueto step 2 (see page 3-19).5. Choose from the following:– If running a single plate, then continue to step 2.– If running the first plate in a series of plates with identical assayconfigurations, then save the plate document as a template (see page 3-24).6. Create a plate document from the template created in step 2 (see page 3-26).7. Configure the document with sample names and plate information(see page 3-28).a. Prepare the allelic discrimination plate or plates and perform thermal cyclingon a designated thermal cycler (see page 4-1).b. Run the allelic discrimination plate or plates on the 7900HT instrument.8. Analyze the run data (see page 5-5).*Steps 2 and 2 can be eliminated by importing the plate document setup informationfrom a tab-delimited text file. See “Importing Plate Document Setup Table Files”on page A-2 for more information.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 3-7DRAFTSeptember 1, 2004 11:39 am, CH_Run.fm