Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Opening the Run DataOpening the Run DataUser AccessRequirementOpen theUn-analyzedPlate DocumentThere is no access requirement. All users can open dissociation curve data that hasbeen saved to the SDS Enterprise Database.Opening a Plate Document File1. Click (or select File > Open).2. In the Look in field, navigate to and select the plate document file.3. Click .The SDS software displays the plate document file.4. Configure the Analysis Settings for each marker as explained below.Opening Un-analyzed Data from the SDS Enterprise Database5. Click (or select File > Open Document from Database).6. In the Plate Query dialog box, configure the Find Plates Matching TheseCriteria table with parameters that correspond to the session of interest.7. Click .8. In the Search Results list, scroll to and select the session that you want toanalyze.9. Click .10. Click when finished.Analyzing the Run DataUser AccessRequirementAnalyzing theRunThere is no access requirement. All users can analyze dissociation curve data that hasbeen saved to the SDS Enterprise Database.The run data from a temperature ramp can be analyzed immediately following thecompletion of the run. For an explanation of how the software manipulates the rawdata, see “Algorithmic Manipulation of Raw Data” on page 6-7.1. Select all wells in the plate grid.The software outlines the selected wells with a black border.2. Choose one of the following:• Select Analysis > Analyze.• Click .The software analyzes the run data and displays the results in the DissociationCurve tab.3. Determine the T m for the dissociation curves displayed within the DissociationCurve tab as explained on page 6-44.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-41DRAFTSeptember 1, 2004 11:39 am, CH_Real-Time.fm