Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Performing a Background RunAnalyzing the Background Data2. Click .The 7900HT instrument begins the background run.Note: Before starting the run, the instrument may pause (up to 15 min) to heatthe heated cover to the appropriate temperature.3. When the background run is complete and the Run Complete dialog boxappears:a. Click to close the dialog box.b. Click and remove the plate from the instrument tray.c. Extract the background component as explained on page 7-19.Extracting theBackgroundIn this procedure, you will extract the calibration values from the background platedocument. After extraction, the SDS software stores the data as part of the calibrationfile located in the Calibration subdirectory of the SDS directory.1. Select Analysis > Extract Background.The software attempts to extract the background signal and displays the successof the extraction in a dialog box.If the software displays the:• Calibration Update Complete dialog box, then the run is successful.Proceed to step 2.The raw spectra read from the Background plate conform to acceptablelimits.• Error dialog box, then the run is unsuccessful. Troubleshoot anddecontaminate the sample block as explained in “Background Runs” onpage 8-9.The software has stopped the extraction because one or more raw spectraexceed 2500 FSU.2. Click (or select File > Save).The software saves the plate document.3. Select File > Close.The software closes the plate document.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-19DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm