Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 2Getting StartedLesson 1: Using Plate DocumentsAbout SDS PlateDocumentsEvery plate run on the Applied Biosystems 7900HT Fast Real-Time PCR Systemrequires the creation of a plate document using the SDS software. A plate documentis a virtual representation of a consumable used to contain samples and reagentsduring a sequence detection run. The software uses the plate document to coordinatethe operation of the instrument (thermal cycling and data collection), to organize andstore the data gathered during the PCR, and to analyze the run data.Table 2-3Plate Document Types of the SDS SoftwareFile Ext. Icon DescriptionSDS 7900HTDocumentSDS 7900HTTemplateDocumentSDS 7900HTMultiple PlateDocument*.sds*.sdt*.sdmPlate Documents are the primary file you will use.They are generated for every kind of experimentand are generally used to run plates.Although optional, plate document templates areuseful as timesaving devices for experiments wheresamples are run on plates with identical assayconfigurations.Multiple plate documents are used to analyze datafrom relative quantification experiments. The use ofmultiple plate documents is discussed in detail inChapter 6, “Analyzing Real-Time Data.”The exercises in this lesson will familiarize you with the use of plate documents.2-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:38 am, CH_GetStart.fm