Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Adding Custom Dyes to the Pure Dye Set4. Apply pure dyes to the custom plate document:a. Select the wells containing the custom dye.b. In the Dyes drop-down list in the Setup tab, select the appropriate dye.The software applies the dye to the selected wells.c. Repeat steps a and b to configure the plate document with any additionalcustom dyes.Dyes drop-down listCustom dye added to selectedwells of the plate document5. Save the custom Pure Dye plate document as a plate document template:a. Click (or select File > Save).b. In the Save dialog box, navigate to Program Files > AppliedBiosystems > SDS 2.2.1 > Templates.The Templates directory appears inside the Look in field. By saving theplate document template to the Templates directory, it becomes availablefrom the Template drop-down list in the New Document dialog box.c. Select Files of type > SDS 7900HT Template Document (*.sdt).d. In the File name field, and enter a name for the plate document template.e. Click . The software saves the plate document as a plate documenttemplate.6. Run the custom Pure Dye plate as explained in “Preparing the Pure Dye Plates”on page 7-22.Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-29DRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm