Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the Instrument5. Click Manual Control to display the Manual Control dialog boxAligningInput Stack 1(ZymarkPosition 4)The alignment of input stack 1 (position 4 in the Zymark Twister Software) is thefirst step in the alignment procedure. This alignment provides the basis for aligningall subsequent stacks on the Plate Handler.1. Place an empty plate into input stack 1(Zymark position 4).2. In the Zymark Twister Software, click position 4.ClickThe Plate Handler arm moves over the input stack.3. Using the Vertical Positioning commands, lower the Plate Handler arm until itis just above the stack.The Vertical Positioning box offers four ways to move the Plate Handler arm:• Move the slider for large increments.• Click inside the slider bar to move the arm in 250 step increments.• Click the lower arrow on the bar to move the arm in 50 step increments.• Click the up or down arrows in the Vertical Adjustment text box to movethe arm in 1-step increments.• Click the Vertical Adjustment text box, enter a value, and press Enter tomove the arm into a specific location.SliderSlider bar (250 steps per click)Down arrow (50 steps per click)Text box arrows (1 step per click)4. Check the rotary position of the Plate Handler arm to confirm that the gripper:• is centered over the stack• will not contact the sides of the stack when lowered7-42 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm