Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 4Operating the Instrument4. When the cover has fully opened, remove the buckets from the centrifuge, thenremove the card holders from the buckets.5. Remove all Low Density Arrays from the buckets by gently lifting them by theircarrier sides.6. Examine the Low Density Arrays to determine whether filling is complete.The amount of cDNA sample or control remaining in the fill reservoirs shouldbe uniform and consistent from reservoir to reservoir.• If there is excess cDNA sample or control remaining in a fill reservoir (asshown below), filling is incomplete. Proceed to the next step.• If a fill reservoir is completely drained (as shown below), it is possible thatsome wells were not filled properly.– The Low Density Array should not be processed further. Use anotherLow Density Array.– Alternatively, you can process the Low Density Array further; however,you should void the results for the affected fill reservoir (sample).7. Evaluate the filling as follows:If all fill reservoirs are...not uniform after the firstcentrifuge cyclenot uniform after theadditional centrifuge cycleThen...the Low Density Array may be centrifuged again in thesame manner as before for 1 additional min.• The Low Density Array should not be processedfurther. Use another Low Density Array.• Alternatively, you can process the Low Density Arrayfurther; however, you should void the results for theaffected fill reservoir (sample).IMPORTANT! Do not exceed 1200 rpm or accumulated centrifugation times ofmore than 3 min. Excessive centrifugation speeds and times may deform theLow Density Array.4-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Basic.fm