Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

Chapter 7Maintaining the InstrumentDecontaminating the Sample BlockWhen to PerformMaterialsRequiredThe following procedure describes how to decontaminate the wells of a sample blockmodule. The procedure eliminates residual PCR-related products, includingfluorescent labeled TaqMan ® probes. Clean the sample block as often as needed.IMPORTANT! If you plan to use a cleaning or decontamination method other thanthe one in this manual, check with Applied Biosystems first to ensure that themethod will not damage the sample block module or the 7900HT instrument.• 10% Sodium hypochlorite (bleach) solution• Isopropanol, 100 percent pure• 5/32 in hex key• Cotton swabsCHEMICAL HAZARD. Sodium hypochlorite (bleach) is aliquid disinfectant that can be corrosive to the skin and can cause skindepigmentation. Please read the MSDS, and follow the handling instructions. Wearappropriate protective eyewear, clothing, and gloves.CHEMICAL HAZARD. Isopropanol is a flammable liquid andvapor. It may cause eye, skin, and upper respiratory tract irritation. Prolonged orrepeated contact may dry skin and cause irritation. It may cause central nervoussystem effects such as drowsiness, dizziness, and headache, etc. Please read theMSDS, and follow the handling instructions. Wear appropriate protective eyewear,clothing, and gloves.Cleaning a LowDensity ArrayBlockThis procedure applies to the sample block on the 7900HT System TaqMan LowDensity Array Upgrade.1. Wipe the surface of the sample block (grey aluminum) with 10% bleach.2. Wipe the sample block three times with distilled water.3. Wipe the sample block with isopropanol, then allow to air dry.WarningCircuitry and connections tothe instrument (Do Not Touch)Well A-1GR20284. Replace the block as explained in “Replacing the Sample Block” on page 7-10.5. Run a background Low Density Array to confirm that the contamination hasbeen removed.7-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideDRAFTSeptember 1, 2004 11:39 am, CH_Maintenance.fm