Applied Biosystems 7900HT Fast Real-Time PCR System and SDS ...

fluorescent, common sources 8-7isolating on the sample block module 8-9contextual menus, using 2-28Control assay 4-11copyingdetectors to a plate document 3-13markers to a plate document 3-15Cover readout (from the Real Time tab) 4-27creatingbackground plate documents 7-17custom pure dye plate documents 7-28detectors 3-11markers 3-14plate documents 2-13, 3-9plate documents from a template 3-26, 4-36pure dye plate documents 7-22C T , See threshold cyclecustom pure dyesadding to the pure dye set 7-27 to 7-29creating a custom pure dye plate 7-27cycle set, adding to a method 3-21Ddata type definitions (exportable) A-17databasesaving plate documents 4-24saving results 5-19, 6-53saving templates 3-25database connection A-22decontaminating the sample block module 7-14 to 7-15defragmenting the hard drive 7-54deleting, steps from a method 3-21detector tasksabout 3-16applying 3-16importing into a plate document A-2detectorsabout 3-11applying to a plate document 3-13copying to a plate document 3-13creating 3-11importing into a plate document A-2tasks, See detector tasksdetermining melting temperatures 6-44diagramdatabase LAN setup 1-23database WAN setup 1-24disconnecting the SDS software 4-30display settings, adjusting 3-24dissociation curveabout 6-38analyzing 6-40 to 6-44definitions of the T m value 6-43procedure checklist 6-22, 6-40programming a temperature ramp 3-23Dissociation Plotabout 6-42exporting as a graphic file A-16exporting data as a text file A-16Eejecting a plate from the 7900HT instrument 4-29,4-44endogenous control 6-20end-point runsabout 5-2allelic discrimination, See allelic discriminationenteringbar code information 2-27, 3-28, 4-36comments 3-11, 3-28sample names into a plate document 3-28Equipment needed 4-12exportable data type definitions A-17exportingdata from a plate document 2-14, A-16plots and views as graphic files A-16External endogenous control assay 4-11Ffinger padscleaning 7-52replacing 7-52fixed-position bar code readeraligning 7-49 to 7-51connections 1-10location 1-7specification 1-7fluorescentcontamination 8-7detection system 1-10fluorogenic probeabout D-2designing B-2 to B-3Ggene expression levelmaximum 6-33minimum 6-33gene expression levels 6-31Gene Expression Plot 6-31Genomic DNA contamination, minimizing 4-11genotypic segregation of datapoints(Allelic Discrimination Plot) 5-8Geometric (Exponential) Phase D-4graph, Gene Expression Profilesample bars 6-31X-axis 6-31Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User GuideIndex-3DRAFTSeptember 1, 2004 11:38 am, 4351684AIX.fm