Four Seasons of Learning and Engaging Smallholder Farmers - icrisat
Four Seasons of Learning and Engaging Smallholder Farmers - icrisat
Four Seasons of Learning and Engaging Smallholder Farmers - icrisat
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Progress in selection <strong>of</strong> Bradyrhizobium strains<br />
At IITA-Ibadan, four soybean genotypes (TGx 1448-2E, TGx 1485-1D, TGx 1838-5E, <strong>and</strong> TGx 1906-<br />
3F) were inoculated with 20 Bradyrhizobium strains in 2008. One milliliter portions <strong>of</strong> broth cultures<br />
were used to inoculate each <strong>of</strong> six seedlings <strong>of</strong> the four genotypes grown in beach s<strong>and</strong> with regular<br />
supply <strong>of</strong> plant nutrient solution. The controls were plus N (70 ppm) <strong>and</strong> non-inoculated control (minus<br />
Rhizobium <strong>and</strong> minus N). Plant cultures were maintained in the greenhouse for 10 weeks (10 June<br />
to 19 August 2008). Soybean genotypes inoculated with Bradyrhizobium strain RAUG1 <strong>and</strong> RAUG2<br />
had the highest shoot nitrogen concentration <strong>and</strong> these figures were significantly higher than that <strong>of</strong><br />
soybean genotypes inoculated with the other strains (Table 11-25). It is important to state that the lower<br />
shoot nitrogen concentration reported for some <strong>of</strong> the genotypes might have been due to dilution effect<br />
associated with larger shoot biomass compared to that <strong>of</strong> soybean genotypes inoculated with RAUG1<br />
<strong>and</strong> RAUG2. The high performing Bradyrhizobium strains have been multiplied <strong>and</strong> the inocula tested<br />
on many more soybean genotypes under field conditions.<br />
Table 11-25: Shoot dry weight <strong>and</strong> nitrogen concentration in the shoot <strong>of</strong> four soybean genotypes inoculated<br />
with 20 Bradyrhizobium strains at IITA-Ibadan.<br />
Bradyrhizobium<br />
strain<br />
Shoot dry weight (g) Nitrogen concentration in shoot (%)<br />
G1 G2 G3 G4 Means G1 G2 G3 G4 Means<br />
RAUG1 19.493 19.443 4.973 5.180 12.272 3.883 4.146 4.013 4.141 4.046<br />
RAUG2 18.487 18.873 6.253 4.387 12.000 4.187 4.139 3.972 3.75 4.012<br />
RACA2 17.273 16.377 6.177 7.310 11.784 3.687 3.499 3.607 3.524 3.579<br />
Irj 2180A 13.133 10.733 13.08 9.697 11.661 3.612 3.970 2.925 3.276 3.446<br />
RACA4 14.033 14.637 8.663 8.077 11.353 3.795 3.496 3.306 3.661 3.565<br />
RACA6 13.807 16.037 8.043 7.490 11.344 3.735 3.426 3.712 3.743 3.654<br />
RACA3 15.330 15.85 6.790 7.183 11.288 3.557 3.638 3.413 3.845 3.613<br />
R.25B 11.610 11.523 9.700 7.883 10.179 3.480 3.781 2.838 3.539 3.410<br />
S(3)PEAT 9.653 6.687 9.487 11.760 9.397 2.673 3.334 3.272 3.317 3.149<br />
RANI 22 7.053 7.830 9.163 6.500 7.637 3.435 3.602 3.211 3.059 3.327<br />
FA3 11.103 6.100 6.650 6.560 7.603 3.641 3.807 3.416 3.417 3.570<br />
TAL169 10.200 8.407 6.577 4.650 7.458 3.778 3.929 2.928 3.536 3.543<br />
USAD4671 9.390 8.157 2.813 8.973 7.333 2.919 2.802 2.028 2.875 2.656<br />
S(I)PEAT 7.083 6.337 6.700 7.613 6.933 3.683 3.818 3.298 3.237 3.509<br />
R.58 10.297 4.007 0.950 7.380 5.658 2.676 2.604 0.927 2.499 2.177<br />
N+ (control) 4.607 5.133 5.300 6.040 5.270 1.290 1.648 1.089 1.310 1.334<br />
MRCP1 1.840 6.893 0.850 10.730 5.078 1.281 2.832 1.326 2.992 2.108<br />
R.31B 2.300 0.913 0.563 11.720 3.874 2.421 1.399 1.029 1.976 1.706<br />
RATG9 1.810 1.637 0.650 5.870 2.492 1.498 1.424 0.997 2.159 1.519<br />
N- (control) 3.287 1.367 1.157 3.087 2.224 1.238 1.127 1.039 1.413 1.204<br />
R.59 1.470 0.817 0.937 2.383 1.402 1.195 1.546 1.364 1.287 1.348<br />
Means 9.679 8.941 5.499 7.165 2.936 3.046 2.558 2.979<br />
SE (G) 0.360 0.050<br />
SE (B) 0.820 0.120<br />
SE (G x B) 1.640 0.240<br />
P (G) ≤ 0.0001 ≤ 0.0001<br />
P (B) ≤ 0.0001 ≤ 0.0001<br />
P (G x B) ≤ 0.0001 ≤ 0.0001<br />
CV (%) 36.210 16.640<br />
G1= TGx 1906-3F, G2= TGx1484-1D, G3= TGx1448-2E, G4= TGx1835-5E<br />
G = Genotype, B = Bradyrhizobium strain, G x B= Genotype × Bradyrhizobium strain<br />
238<br />
<strong>Engaging</strong> <strong>Smallholder</strong> <strong>Farmers</strong> | Tropical Legumes II Project