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Preface - kmutt

Preface - kmutt

KMUTT Annual Research

KMUTT Annual Research Abstracts 2005 holding solution did not appear to have a negative effect on petal wilting, opened flower bud, and vase life of orchid flower. IC-255 SURVIVAL OF SEEDED Escherichia coli O157:H7 AND Salmonella ON SELECTED FRESH HERBS Pongphen Jitareerat, Amy Simonne, Wei-Yea Hsu APEC Symposium on Assuring Quality and Safety of Fresh Produce, August 1-3, 2005, Radisson Hotel, Bangkok, Thailand Fresh herbs are often implicated in foodborne illness outbreaks in the US. This study examined the survival of seeded foodborne pathogens (E. coli O157:H7 and Salmonella spp.) on different fresh herbs at refrigerated temperature (4°C, 96% RH). We purchased fresh herbs (basil, chive, cilantro, oregano, parsley and rosemary) packed in individual clamshells from a commercial farm, and we inoculated them with cocktails of three nalidixic acid-resistant strains of E. coli O157:H7 (204P, 505B, 301C) and three Salmonella serotypes (S. typhimurium, S. mission, S. enteritidis) at the concentration of approximately 2.0 x 10 6 cfu/g fresh weight. We checked the microbial populations of the inoculated herbs after 1, 5, 11, 16, 19 and 24 days of storage. For both pathogens, the significant decrease (p< 0.0001) in cfu/g occurred between day 1 and day 5 (0.5 log cycle), but thereafter, the decrease was not significant. From our results, it appeared that both pathogens remained the highest in cilantro and lowest in rosemary (p< 0.0001). Although we terminated the storage of cilantro, basil, and chive at 19 days due to poor quality, significant numbers of both pathogens survived in the fresh herbs through the 24 days storage. This result reenforces the concept that once contaminated, the microbes could persist in the fresh herbs. This result also suggested that survival of these two pathogens may be affected by the type of herbs. IC-256 EFFECT OF CHITOSAN FOR PRE- HARVEST AND POST-HARVEST DISEASES CONTROL OF RAMBUTAN CV. RONG REAN Pongphen Jitareerat, Yupin Kasinkaseampong, Prapaporn Chanthanumaut, Dusit Pradabsri APEC Symposium on Assuring Quality and Safety of Fresh Produce, August 1-3, 2005, Radisson Hotel, Bangkok, Thailand 165 Chitosan was used to control pre-harvest diseases of rambutan cv. Rong Rean in the orchard at Chumporn province, Thailand. Rambutan trees were sprayed with chitosan at the concentration of 0, 0.03, 0.06, 0.09 and 0.12 % (w/v) every two weeks for three months during flower bloom stage and 1,000 ppm of benomyl was used as a positive control. Preharvest diseases (powdery mildew, algal spot, and sooty mold), post-harvest diseases (fruit rot), and fruit quality of rambutan were investigated in this experiment. The results showed that a decrease in powdery mildew was affected by 0.12 and 0.09% chitosan respectively but this was not caused by the induction of plant defense mechanisms, or peroxidase activity. Rambutan fruits were harvested from chitosan treated rambutan trees and fruit rot and fruit quality were determined after fruits were stored at 13°C. Spraying chitosan at 0.12% improved rambutan quality by increasing fresh weight and firmness and it did not appear to have a negative effect on spintern color or total soluble solid. Moreover, 0.12% chitosan treated fruits showed the lowest amount of fruit rot (13%) while the control fruits and benomyl treated fruits had 23% and 34% rot respectively. Peroxidase activity in peel of rambutan was increased by 0.09% chitosan treatment but not by 0.12% chitosan treatment. These results imply that the effect of chitosan treatment on fruit rot of rambutan may not be associated with peroxidase activity. IC-257 DETECTION OF LATENT INFECTION OF Colletotrichum gloeosporioides, CAUSAL AGENT OF ANTHRACNOSE DISEASE IN RAW MANGO FRUITS BY POLYMERASE CHAIN REACTION Pongphen Jitareerat, Somsiri Sangchote, Chalermchai Wongs-Aree The 2 nd Asian Conference on Plant Pathology, June 25-28, 2005, National University of Singapore, Singapore Anthracnose disease is a major problem for mangoes meant for exporting in many countries due to the invasion of Colletotrichum gloeosporioides in all mango tissues. The initial infection occurs during flower bloom and then exists as a latent infection in mature greenmangoes. The disease symptom thereafter appears when the fruits are ripening. A rapid International Conference

166 method to detect the latent C. gloeosporioides infection in green mangoes is becoming necessary for proper assessment of the decay and loss of mangoes when they arrive in the terminal market, and also to provide suitable methods for disease control. Polymerase chain reaction (PCR) is often used to detect and identify plant pathogens using highly specific oligonucleotide primers. In this experiment, Cglnt-ITS4 primers amplifying the conserve regions of the 25S-28S rRNA gene specific only on genomic DNA of Colletotrichum sp. but not on Phomopsis sp., Botryodiplodia theobromea, and Aspergillus niger, The ITS1-4 (ITS1-ITS2 and 5.8 rRNA gene), ITS4-5 (the region between small-large nuclear rDNA and 5.8 rDNA) and CAP20 (appressorium forming gene) primers showed the specific amplification on those genes of Colletotrichum sp., giving amplified fragment sizes of 590, 590 and 610 bp, respectively but also amplified unknown DNA regions from other fungi. In contrast, CgmPG2 primer (designed from polygalacturonase gene) was not specific for Colletotrichum. The sensitivity of the PCR, the minimum amounts of fungal genomic DNA needed, was evaluated. The results revealed that the lowest amount of fungal DNA which could be amplified was 1 fg by the ITS1-4 and ITS4-5 primers and 10 pg by Cglnt- ITS1 primer. Latent infection of C. gloeosporioides in raw mangoes without disease symptom and ripen mangoes with disease symptom was detected. The results showed that Cglnt-lts4 and ITS1-ITS4 primers could use to detect the latent infection from both samples. On the other hand, amplification by ITS4-ITS5 primers surprisingly showed on extra DNA band from the raw mango samples. IC-258 EFFECT OF CHITOSAN ON CONTROLLING OF ANTHRACNOSE DISEASE IN MANGOES CV. NAM DOK MAI Sudkanung Paumchai, Pongphen Jitareerat, Sirichai Kanlayanarat, Somsiri Sangchote The 2 nd Asian Conference on Plant Pathology, June 25-28, 2005, National University of Singapore, Singapore Effect of chitosan on mycelial growth and spore germination of Colletotrichum gloeosporioides was investigated on Potato Dextrose Agar (PDA) containing 0, 0.5, 1.0 and 2.0% (w/v) chitosan dissolved in 0.5% (w/v) acetic acid. The results revealed that 1.5 and KMUTT Annual Research Abstracts 2005 2,0% chitosan were the best concentration for inhibiting the mycelial growth and spore germination, while complete inhibition was found on the medium containing 0.5% acetic acid. Effect of the chitosan coating on artificially C. gloeosporioides inoculated mangoes showed that the best concentration of chitosan to control anthracnose disease was at 0.5 and 1.0%. These concentration could stimulate chitinase and beta- 1,3-glucanase activities which were involved in the plant defense mechanism in chitosan treated mango fruits. Additionally, the chitosan coating on mangoes could delay rippening, reduce respiration rates, ethylene production, weight loss, ascorbic acid and total titratable acidity but not maintain the firmness of the mangoes. IC-259 EFFECT OF ETHYLENE AND 1- METHYLCYCLOPROPENE ON FIBER AND LIGNIN CONTENT OF ASPARAGUS Nantipa Keawpet, Pongphen Jitareerat, Sirichai Kanlayanarat APEC Symposium on Assuring Quality and Safety of Fresh Produce, August 1-3, 2005, Radisson Hotel, Bangkok, Thailand Asparagus (Asparagus officinalis) spears cv. 'Brock Improve' were fumigated with 500 ppb 1-MCP for 12 hr alone or in combination with 100 ppm ethylene exposure for 3 hr. Ethylene was applied either before or after 1-MCP treatment. Untreated spears served as control. Treatment and subsequent storage conditions were 20°C and 90% RH. 1-MCP reduced the shear-press force indicating lower degree of toughening of the spears compared to that of the untreated control. Ethylene exposure prior to 1-MCP treatment eliminated the 1-MCP effect and accelerated toughening relative to the control. Correspondingly, fiber and Iignin contents and peroxidase activity were lowest with 1-MCP alone and highest with ethylene exposure prior to 1-MCP treatment. When ethylene was applied following 1-MCP treatment, the 1-MCP effect prevailed initially but later decreased. In this treatment, texture changes did not compare well with changes in fiber and Iignin contents, suggesting the involvement of other cell wall and carbohydrates components in texture quality of asparagus. IC-260 INHIBITON OF FIBER FORMATION AND LIGNIFICATION OF ASPARAGUS BY 1- METHYLCYCLOPROPENE International Conference

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    CONTENTS Page Preface International

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    National Journals

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    54 นอยกวาแบบต

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    56 (Least Square Matching Method)

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    58 อุณหภูมิ 30 แ

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    60 สเปกตรัม จา

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    62 ขอมูลดานทร

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    64 22.8 มาตรฐานเป

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    66 2545 NJ-033 EFFECTS OF ELEVATED

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    68 เปลือกที่ม

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    70 ของการดูดซ

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    72 การสกัดดวย

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    74 collection of authentic texts th

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    76 รอยละ 32 มีสา

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  • Page 214: National Conferences
  • Page 217 and 218: 210 จอมเทียนปา
  • Page 219 and 220: 212 การสกัดสาร
  • Page 221 and 222: 214 ไดแก อุณหภู
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    216 โดยกลไกของ

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    218 เคลือบเมื่

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    220 สําเริง จัก

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    222 ลดเวลาตลอด

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    224 อุณหภูมิสู

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    226 NC-043 การวิเคร

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    228 เกิดพฤติกร

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    230 เดียว เพื่อ

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    232 มากนอยเพีย

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    234 หลักที่พบใ

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    236 NC-073 การออกแบ

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    238 บทความนี้น

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    240 นําเอาเวคเ

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    242 งานวิจัยนี

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    244 การอบแหงลด

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    246 แยกเฟสต่ํา

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    248 กลบ โดยเปรี

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    250 นํามาใช คือ

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    252 วิเคราะหหา

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    254 NC-123 การวิเคร

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    256 NC-129 เครื่องผ

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    258 สามารถขยาย

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    260 ครั้งที่ 43, 1-

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    262 calculated with the use of the

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    264 Version 1.3.1 และ Softwar

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    266 ขนไกได จากก

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    268 โครงสรางขอ

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    270 การปลูกพืช

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    272 ระดับพึงพอ

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    274 ของแกสโซฮอ

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    276 1) สภาพทั่วไ

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    278 อนุปริญญา แ

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    280 อาชีวศึกษา

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    282 กระทบตอคุณ

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    284 ตั้งกลไกแข

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    286 นุชจรินทร เ

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    288 โดยรวมผูบร

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    290 แนนกระแสไฟ

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    292 NC-222 ผลงานตีพ

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    294 NC-227 เตาเผาไห

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    296 จําเพาะรวม

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    298 NC-238 จลนพลศาส

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    300 ทนงเกียรติ

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    302 ดีเซลนั้นม

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    304 NC-254 การใชเทค

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    306 อาคารศูนยก

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    308 กระบวนการร

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    310 บรรยากาศขอ

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    312 metal ions on 2-AP biosyntheis

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    314 total clones while the ethanol

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    316 การประชุมท

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    318 BioThailand (The 16 th Annual M

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    320 สูงที่สุดพ

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    322 นัยสําคัญท

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    324 ความเขมขนร

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    326 ปลูกแบบพรา

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    328 นี้ทําใหทร

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    330 ดุลชาติ มาน

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    332 ไมโครเมตร ส

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    334 In this study, we collected seq

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    336 กนกรัตน นาค

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    338 การพัฒนาสื

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    340 one of the causes of difficulty

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    342 ทวีรัตน วิจ

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    344 program, Cytoscape. This model

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    346 production demand. Nevertheless

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    348 อุลตราไวโอ

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    350 นี้อยูในชว

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    352 เพื่อนําสว

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    354 trichloroacetic acid (TCA) ม

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    356 ทรัพยากรที

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    358 ในประเทศสห

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    360 8-9 ธันวาคม 2548,

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    362 ซึ่งเกิดขอ

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    364 NC-391 การวิเคร

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    366 การประชุมว

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    368 2547) พบวามหาว

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    370 กนกพร ลีลาเ

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    Authors Index

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    376 จงจิตร หิรั

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    378 ธ ธนธร ทองส

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    380 พยุงศักดิ์

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    382 วรรณพ วิเศษ

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    384 สุดารัตน จิ

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    386 อุลาวัณย กุ

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    388 138, 141, 142 Jarunya Narangaja

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    390 Panida Kongsawadworakul 191 Pan

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    392 Sorakrich Maneewan 124, 136 Sor

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    394 KMUTT Annual Research Abstracts

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