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Preface - kmutt

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KMUTT Annual Research Abstracts 2005<br />

Nantipa Keawpet, Pongphen Jitareerat,<br />

Sirichai Kanlayanarat<br />

APEC Symposium on Assuring Quality and<br />

Safety of Fresh Produce, August 1-3, 2005,<br />

Radisson Hotel, Bangkok, Thailand<br />

Asparagus (Asparagus officinalis)<br />

spears cv. 'Brock Improve' were fumigated with<br />

0, 50, 250, 500 or 1000 ppb 1-MCP for 12 hr at<br />

20°C and then held at 20°C with 90% RH. Fiber<br />

and lignin contents increased with storage<br />

corresponding to the increasing toughness of the<br />

spears indicated by increasing shear-press force.<br />

1-MCP reduced fiber and lignin formation and<br />

toughening. Such inhibitory effect of 1-MCP<br />

increased with increasing concentration of up to<br />

500 ppb. The effectiveness of 1-MCP declined<br />

at higher concentration of 1,000 ppb. Peroxidase<br />

activity followed similar trend as fiber and<br />

lignin content. It was also inhibited by 1-MCP<br />

which was most effective at 500 ppb. However,<br />

peroxidase activity did not coincide with lignin<br />

content during the first 2 days of storage.<br />

IC-261 ANTIFUNGAL ACTIVITY OF<br />

CINNAMON (Cinnamonmum zeylanicum)<br />

EXTRACT ON BANANA CROWN ROTTING<br />

FUNGI<br />

Nang Kyu Kyu Win, Pongphen Jitareerat,<br />

Sirichai Kanlayanarat<br />

APEC Symposium on Assuring Quality and<br />

Safety of Fresh Produce, August 1-3, 2005,<br />

Radisson Hotel, Bangkok, Thailand<br />

Crown rot is the important postharvest<br />

disease of banana caused by complex fungi; C.<br />

musae, Lasiodiplodia theobromae, and<br />

Fusarium spp. are ubiquitous in postharvest<br />

storage. Due to the consumer concern on<br />

fungicide residue therefore, alternative methods<br />

for controlling of postharvest diseases were<br />

required. The antifungal activity of cinnamon<br />

extracts on these pathogens was observed in<br />

vitro at room temperature. Inhibition of conidial<br />

germination and mycelial growth was<br />

investigated on potato dextrose agar with 0, 100,<br />

500, 1,000, 5,000 and 10,000 ppm of cinnamon<br />

extract in compared with 750 ppm carbendazim.<br />

The results revealed that cinnamon extracts at<br />

5000 ppm completely arrested the conidial<br />

germination and growth of all pathogenic fungi.<br />

Conidia of L. theobromae was the most sensitive<br />

to cinnamon extract at the lowest concentration<br />

(100 ppm) with 18.33% of inhibition while C.<br />

167<br />

musae and Fusarium spp. were 46.67 and<br />

78.33% respectively. However, mycelial growth<br />

of Fusarium spp. was affected by 1,000 ppm<br />

with the inhibition percent of 75.71% but<br />

77.18% and 33.35% for C. musae and L.<br />

theobromae. The ED 50 values of cinnamon<br />

extracts were 588, 1,950, and 692 ppm for<br />

mycelial growth of C. musae, L. theobromae and<br />

Fusarium spp. and were 91, 50 and 758 ppm for<br />

their conidia. This study indicated that the ED 50<br />

value of cinnamon extracts for mycelial growth<br />

was higher than that of conidia germination,<br />

excepted Fusarium spp. and the cinnamon<br />

extract at 5,000 ppm has a potential to be used as<br />

biological control to reduce crown rot disease of<br />

banana.<br />

IC-262 PREHARVEST CHITOSAN SPRAYING<br />

ON LEAF SPOT DISEASE AND GROWTH OF<br />

ORCHID (Dendrobium missteen)<br />

Nang Kyu Kyu Win, Pongphen Jitareerat,<br />

Sirichai Kanlayanarat<br />

APEC Symposium on Assuring Quality and<br />

Safety of Fresh Produce, August 1-3, 2005,<br />

Radisson Hotel, Bangkok, Thailand<br />

Leaf spot disease on mature orchid<br />

plants caused by Colletotrichum gloeosporioides<br />

is commonly a problem of flower quality.<br />

Avoidance of chemical residue on the<br />

environment and human safety, natural<br />

substance as chitosan was used to control leaf<br />

spot disease in this experiment. Chitosan was<br />

sprayed on orchid plants which had leaf spot<br />

disease at the concentration of 0, 0.6, 1.2, 1.8<br />

and 2.4% every week for 10 weeks under natural<br />

condition. Disease development, quality of<br />

flower and growth of shoot were assessed during<br />

preharvesting. The results revealed that chitosan<br />

spraying significantly reduced leaf spot disease<br />

severity at the concentration of 1.2, 1.8 and<br />

2.4% compared to control and 0.6% chitosan<br />

sprayed plants. However, quality characteristic<br />

such as number of flowers, flower size and also<br />

growth of plant (new shoot emergence and<br />

length of shoots) were not significant different<br />

among treatments excepted the shoot length of<br />

1.8% and 2.4% chitosan sprayed plants were<br />

significantly higher than that of other treatments.<br />

The results suggested that preharvest spraying of<br />

chitosan can be used to reduce leaf spot disease<br />

severity on mature orchid plants although it<br />

could not influence on the quality of flowers and<br />

growth of orchid plants.<br />

International Conference

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