, ~/;'C)U -~ 'v' J L,Lt.~·~,j· ., "-.f' BIBLIOGRAPHIE 110
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Voshinari, K. lino, K. Sonoki, M. Iwase, and M. Fujishima. Peroxisome proliferator-activated receptor gamma1 (PPARy1) expresses in rat mesangial cells and PPARy agonists modulate its differentiation. Bioehim Biophys Aeta 1497: 148-154, 2000. 6. Asou, H., W. Verbeek, E. Williamson, E. Eistner, T. Kubota, N. Kamada, and H. P. Koeff<strong>le</strong>r. Growth inhibition of myeloid <strong>le</strong>ukemia cells by troglitazone, a ligand for peroxisome proliferator activated receptor gamma, and retinoids. Int J Oneo/15: 1027-1031, 1999. 7. Auboeuf, D., J. Rieusset, L. Fajas, P. Vallier, V. Freing, J. Riou, M. Lavil<strong>le</strong>, J. Auwerx, and H. Vidal. Tissue distribution and quantification of the expression of peroxisome proliferator-activated receptors and liver X receptor-ex, in humans : no alterations in adipose tissue and NIDDM patients. Oiabetes 46: 1319-1327, 1997. 8. Aviv, H., and P. Leder. 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AVERTISSEMENT Ce document numéris
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Je remercie chaleureusement Monsieu
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VIII. ROLES DES PPAR DANS CERTAINES
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EXPRESSION DES PPAR AU NIVEAU DE LA
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A mon père, A mon beau-père,
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AINS : non steroid anti inflammator
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Les récepteurs activés par les pr
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CARACTERES GENERAUX SUR LE COLON F
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Se SEMAINE COUPE SAGnTAtB /' Ile SE
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allg le colique droit colon transve
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œlltllê ~Uc:iforme ' Figure 4 : S
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des glandes tubulaires. Les espaces
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La voie de transduction Wnt/Wingles
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l'homologue de TCF chez la Drosophi
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conduisent à des tumeurs desmoïde
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. facteurs transcriptionnels interv
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LES RECEPTEURS ACTIVES PAR LES PROL
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- un domaine E qui intervient dans
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III. DISTRIBUTION TISSULAIRE DES PP
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hétérodimères PPARlRXR se lient
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iW! 1341Oj)§ 16"q~xy,.'\12:,14 prO
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facteur de croissance par la voie M
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VI. INTERVENTION DES PPAR DANS LA D
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les fonctions endothéliales. Et-1
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c. PPAR et autres processus néopla
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CANCERS DU COLON ET PPAR 1. LES CAN
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Le terme de tubuleux est employé l
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musculeuse qui peut être dépassé
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La stadification tumoraux Le degré
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ganglions régionaux envahis. Leur
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éséquées dans le même temps op
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tumorale. Si les cancers colorectau
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colorectaux chez le rat ou la souri
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PPAR ~/û est également impliqué
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Les PPAR sont présents dans le col
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MATERIELS ET METHODES 1. MODELES BI
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puis retourné à l'aide d'une fine
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Dosage de l'activité de l'acyl GoA
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tlJ e SSGSFGFTEVQV T D 2" EIF 4]' P
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kDa A B -. - - ....... 94- 64- 43-
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MONTAGES LEGENDES • • antigène
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ensuite contre-colorée à l'hémat
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La révélation des complexes antig
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les cellules Caco-2 sont lysées en
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solution d'acétate de sodium 0,5 M
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ARN Sondes radioactives / hybridati
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Amorces sens Amorces antisens Réf
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pBSKS+. pBSK+/hPPARa, PBSKS+/hPPARy
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étudiée (figure 20). Nous avons r
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PPARa PPARB Amorces sens (5' vers 3
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Taq 1 coupe uniquement le fragment
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PCR semi-quantitative Une technique
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Amorces sens Amorces anti-sens Réf
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EXPRESSION DES PPAR AU COURS DU DEV
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Figure 21 : Etude par immunocytochi
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Figure 22: Etude par immunocytochim
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Figure 23: Etude par immunocytochim
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@ 12345678 404 pb- +-G3PDH 242 pb-
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Au niveau de l'intestin, quel que s
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Enzymes Activités spécifiques 5 j
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@ Q) "0 Cti:l- .n 115 ~ ~ ~.~ CIl .
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Figure 27 : Mise en évidence de l'
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PPARa PPARy PPARy (commerciale) Fig
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même, lorsque l'anticorps primaire
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Caco-2 5 10 15 TA IR --288 -188 Fig
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COX-1 COX-1 Caco-2 Caco-2 intestin
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o ~- caténine Caco-2 !Tissu adipeu
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- Page 217 and 218: 148 neoplasia. STRUCTURAL ORGANIZAT
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C. Huill et al. / Biology of the Ce
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C. Huin et al. 1 Biology of the CeU
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C. Huin et al. / Biology of the Cel
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C. Hlli" el al./ Bialag)' of/Ile Ce
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C. Huin et al. 1 Biology ofthe Cell
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C. Huill et al. 1 Biology of the Ce
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FACULTE DES SCIENCES & TECHNIQUES S
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Peroxisome proliferator-activated r