371. Xu, H. E., M. H. Lambert, V. G. Montana, D. J. Parks, S. G. Blanchard, P. J. Brown, D. D. Sternbach, J. M. Lehmann, G. B. Wisely, T. M. Willson, S. A. Kliewer, and M. V. Milburn. Mo<strong>le</strong>cular recognition of fatty acids by peroxisome proliferator-activated receptors. Mol <strong>Ce</strong>l! 3: 397-403, 1999. 372. Xu, L., C. K. Glass, and M. Rosenfeld. Coactivator and corepressor comp<strong>le</strong>xes in nuc<strong>le</strong>ar receptorfunction. CurrOpin GenetOev9: 140-147, 1999. 373. Yamamoto, H., S. Kishida, M. Kishida, S. Ikeda, S. Takada, and A Kikushi. Phosphorylation of axinn a Wnt signal negative regulator, by glycogen synthase kinase-3~ regulates its stability. J Biol Chem 247: 10681-10684, 1999. 374. Yanase, T., T. Yashiro, K. Takitani, S. Kato, S. Taniguchi, R. Takayanagi, and H. Nawata. Differentiai expression of PPAR gamma1 and gamma2 isoforms in human adipose tissue. Biochem Biophys Res Commun 233: 320-324,1997. 375. Yang-Snyder, J., J. lilier, J. Brown, C. Lai, and R. Moon. A frizz<strong>le</strong>d homolog functions in a vertebrate Wnt signaling pathway. Curr Biol 6: 1302-1306, 1996. 376. Ye, H., T. Kelly, U. Samadani, L. Lim, S. Rubino, D. Overdier, K. Roebuck, and R. Costa. Hepatocyte nuc<strong>le</strong>ar factor 3/forkhead Homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues. Mol <strong>Ce</strong>l! Biol 17: 1626-1641, 1997. 377. Yee, L. D., C. L. K. Sabourin, L. Liu, H. M. Li, P. J. Smith, V. Seewaldt, and D. A. Kniss. Peroxisome proliferator-activated receptor y activation in human breast cancer. Int J Onco/15: 967- 973,1999. 378. Yu, G. S., Y. C. Lu, and T. Gulick. Co-regulation of tissue-specifie alternative human carnitine palmitoyltransferase I~ gene promoters by fatty acid enzyme substrate. J Biol Chem 273: 32901 32909, 1998. 379. Yu, K., W. Bayona, C. B. Kal<strong>le</strong>n, H. P. Harding, C. P. Ravera, G. MacMahon, M. Brown, and M. A. Lazar. Differentiai activation of peroxisome proliferator-activated receptors by eicosanoids. J Biol Chem 270: 23975-23983, 1995. 380. Zhang, 8., J. Berger, G. Zhou, A. Elbrecht, S. Biswas, S. White-Carrington, D. Szalkowski, and D. E. Mol<strong>le</strong>r. Insulin and mitogen-activated protein kinase-mediated phosphorylation and activation of peroxisome proliferator-activated receptor gamma. J Biol Chem 271: 31771-31774, 1996. 381. Zhang, B., S. L. Marcus, F. G. Sajjadi, K. Alvares, J. K. Reddy, S. Subramani, R. A. Rachubinski, and J. P. Capone. Identification of a peroxisome proliferator-responsive e<strong>le</strong>ment upstream of the gene encoding rat peroxisomal enoyl-coA hydratase/3-hydroxyacyl-coA dehydrogenase. Proc. Nat!. Acad. Sei. USA 89: 7541-7545, 1992. 382. Zhang, L. Gene expression profi<strong>le</strong>s in normal and cancer cells. Science 276: 1268-1272, 1997. 383. Zhou, J., K. Wilson, and J. Medh. Genetic analysis of four novel peroxisome proliferator activated receptor-y splice variants in monkey macrophages. Biochem Biophys Res Commun 293: 274-283, 2002. 384. Zhou, Y. C., and D. J. Waxman. Cross-talk between Janus Kinase-Signal Transducer and Activator of Transcription (JAK-STAT) and peroxisome proliferator-activated receptor-alpha (PPARalpha) signaling pathways. Growth hormone inhibition of PPARaipha transcriptional activity mediated by Stat5b. J Biol Chem 274: 2672-2681,1999. 385. Zhu, X., M. Ohtsubo, R. Bohmer, J. Roberts, and R. Assoian. Adhesion dependent cell cyc<strong>le</strong> progression linked to the expression of cyclin D, activation of cyclin E-cdk2, and phosphorylation of the retinoblastoma protein. J <strong>Ce</strong>l! Biol 133: 391-403, 1996. 386. Zhu, Y., K. Alvares, Q. Huang, M. S. Rao, and J. K. Reddy. Cloning of a new member of the peroxisome proliferator-activated receptor gene family from mouse liver. J. Biol. Chem. 268: 26817 26820, 1993. 387. Zhu, Y., C. Qi, S. Jain, M. S. Rao, and J. K. Reddy. Isolation and characterization of PBP, a protein that interacts with peroxisome proliferator-activated receptor. J Biol Chem 272: 25500-25506, 1997. 388. Zweibaum, A, M. Laburthe, E. Grasset, and D. Louvard. Use of cultured celllines in studies of int<strong>est</strong>inal cell differentiation and function. Handbook of Physiology 4: 223-255, 1991. 389. Zweibaum, A, N. Triadou, M. Kedinger, M. Augeron, S.Robine-Leon, M. Rousset, and K. Haffen. Sucrase-isomaltase, a marker of fetal and malignat epithelial cells of the colon. Int J Cancer 1983: 407-412,1983. 128
~. ' , \ .•1 . ·r·'··.· .~ ,"., PUBLICATIONS 129
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AVERTISSEMENT Ce document numéris
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Je remercie chaleureusement Monsieu
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VIII. ROLES DES PPAR DANS CERTAINES
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EXPRESSION DES PPAR AU NIVEAU DE LA
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A mon père, A mon beau-père,
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AINS : non steroid anti inflammator
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Les récepteurs activés par les pr
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CARACTERES GENERAUX SUR LE COLON F
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Se SEMAINE COUPE SAGnTAtB /' Ile SE
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allg le colique droit colon transve
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œlltllê ~Uc:iforme ' Figure 4 : S
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des glandes tubulaires. Les espaces
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La voie de transduction Wnt/Wingles
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l'homologue de TCF chez la Drosophi
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conduisent à des tumeurs desmoïde
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. facteurs transcriptionnels interv
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LES RECEPTEURS ACTIVES PAR LES PROL
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- un domaine E qui intervient dans
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III. DISTRIBUTION TISSULAIRE DES PP
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hétérodimères PPARlRXR se lient
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iW! 1341Oj)§ 16"q~xy,.'\12:,14 prO
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facteur de croissance par la voie M
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VI. INTERVENTION DES PPAR DANS LA D
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les fonctions endothéliales. Et-1
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c. PPAR et autres processus néopla
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CANCERS DU COLON ET PPAR 1. LES CAN
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Le terme de tubuleux est employé l
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musculeuse qui peut être dépassé
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La stadification tumoraux Le degré
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ganglions régionaux envahis. Leur
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éséquées dans le même temps op
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tumorale. Si les cancers colorectau
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colorectaux chez le rat ou la souri
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PPAR ~/û est également impliqué
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Les PPAR sont présents dans le col
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MATERIELS ET METHODES 1. MODELES BI
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puis retourné à l'aide d'une fine
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Dosage de l'activité de l'acyl GoA
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tlJ e SSGSFGFTEVQV T D 2" EIF 4]' P
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kDa A B -. - - ....... 94- 64- 43-
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MONTAGES LEGENDES • • antigène
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ensuite contre-colorée à l'hémat
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La révélation des complexes antig
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les cellules Caco-2 sont lysées en
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solution d'acétate de sodium 0,5 M
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ARN Sondes radioactives / hybridati
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Amorces sens Amorces antisens Réf
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pBSKS+. pBSK+/hPPARa, PBSKS+/hPPARy
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étudiée (figure 20). Nous avons r
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PPARa PPARB Amorces sens (5' vers 3
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Taq 1 coupe uniquement le fragment
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PCR semi-quantitative Une technique
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Amorces sens Amorces anti-sens Réf
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EXPRESSION DES PPAR AU COURS DU DEV
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Figure 21 : Etude par immunocytochi
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Figure 22: Etude par immunocytochim
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Figure 23: Etude par immunocytochim
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@ 12345678 404 pb- +-G3PDH 242 pb-
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Au niveau de l'intestin, quel que s
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Enzymes Activités spécifiques 5 j
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@ Q) "0 Cti:l- .n 115 ~ ~ ~.~ CIl .
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Figure 27 : Mise en évidence de l'
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PPARa PPARy PPARy (commerciale) Fig
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même, lorsque l'anticorps primaire
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Caco-2 5 10 15 TA IR --288 -188 Fig
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COX-1 COX-1 Caco-2 Caco-2 intestin
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o ~- caténine Caco-2 !Tissu adipeu
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ARN totaux ARN Poly A+ PPARy 51015
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û) cr: ~ « .- ll.. ~ ll.. .~ .0 Q
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RT-PCR Caco-2 (jours de culture) Co
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migration électrophorétique. La q
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l'activité enzymatique de AOX et c
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RT-PCR HT29 (jours de culture) comp
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La protéine PPARy1, contribuent en
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Caco-2 ft , t. "l PPARy cytochrome
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EXPRESSION DES PPAR AU NIVEAU D'ADE
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- Page 167 and 168: III. DISCUSSION a. Expression de PP
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- Page 175 and 176: dans du TBE (figure 48). Le produit
- Page 177 and 178: DISCUSSION GENERALE 102
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- Page 185 and 186: même coupe. Les résultats obtenus
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- Page 189 and 190: 46. Chevalier, S., and R. A Roberts
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- Page 209 and 210: PPARs and Human Fetal Digestive Tra
- Page 211 and 212: PPARs and Human Fetal Digestive Tra
- Page 213 and 214: PPARs and Human Fetal Digestive Tra
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- Page 217 and 218: 148 neoplasia. STRUCTURAL ORGANIZAT
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- Page 221 and 222: 152 hydrolyses triglycerides presen
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