26 C. Huin et al. / Bi%gy ofthe <strong>Ce</strong>l! 94 (2002) 15-27 References Altiok, S., Xu, M., Spiegelman, B.M., 1997. PPARy induces cell cyc<strong>le</strong> withdrawal: inihibition of E2FIDP DNA-binding activity via downregnlation of PP2A Genes Dev. Il, 1987-1998. Auboeuf, D., Rieusset, l, Fajas, L., Vallier, P., Freing, v., Riou, J.P., Lavil<strong>le</strong>, M., Staels, B., Auwerx, J., Vidal, H., 1997. Tissue distribution and quantification of the expression of peroxisome proliferatoractivated receptors and liver X receptor-alpha in humans: no alteration in adipose tissue of obese and NIDDM patients. Diabetes 46, 1319-1327. Aviv, H., Leder, P., 1972. Purification of biologically active protein globin messenger RNA by chromatography on oligothymidylic acid--œllulose. Proc. Natl. Acad. Sci. USA 69, 1408-1412. Baudhuin, S.B., Beaufay, H., Rahman-Li, Y., Sellinger, O.Z., Wattiaux, R, Jacques, P., de Duve, C., 1964. 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AVERTISSEMENT Ce document numéris
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Je remercie chaleureusement Monsieu
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VIII. ROLES DES PPAR DANS CERTAINES
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EXPRESSION DES PPAR AU NIVEAU DE LA
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A mon père, A mon beau-père,
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AINS : non steroid anti inflammator
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Les récepteurs activés par les pr
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CARACTERES GENERAUX SUR LE COLON F
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Se SEMAINE COUPE SAGnTAtB /' Ile SE
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allg le colique droit colon transve
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œlltllê ~Uc:iforme ' Figure 4 : S
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des glandes tubulaires. Les espaces
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La voie de transduction Wnt/Wingles
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l'homologue de TCF chez la Drosophi
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conduisent à des tumeurs desmoïde
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. facteurs transcriptionnels interv
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LES RECEPTEURS ACTIVES PAR LES PROL
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- un domaine E qui intervient dans
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III. DISTRIBUTION TISSULAIRE DES PP
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hétérodimères PPARlRXR se lient
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iW! 1341Oj)§ 16"q~xy,.'\12:,14 prO
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facteur de croissance par la voie M
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VI. INTERVENTION DES PPAR DANS LA D
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les fonctions endothéliales. Et-1
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c. PPAR et autres processus néopla
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CANCERS DU COLON ET PPAR 1. LES CAN
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Le terme de tubuleux est employé l
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musculeuse qui peut être dépassé
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La stadification tumoraux Le degré
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ganglions régionaux envahis. Leur
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éséquées dans le même temps op
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tumorale. Si les cancers colorectau
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colorectaux chez le rat ou la souri
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PPAR ~/û est également impliqué
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Les PPAR sont présents dans le col
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MATERIELS ET METHODES 1. MODELES BI
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puis retourné à l'aide d'une fine
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Dosage de l'activité de l'acyl GoA
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tlJ e SSGSFGFTEVQV T D 2" EIF 4]' P
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kDa A B -. - - ....... 94- 64- 43-
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MONTAGES LEGENDES • • antigène
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ensuite contre-colorée à l'hémat
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La révélation des complexes antig
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les cellules Caco-2 sont lysées en
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solution d'acétate de sodium 0,5 M
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ARN Sondes radioactives / hybridati
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Amorces sens Amorces antisens Réf
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pBSKS+. pBSK+/hPPARa, PBSKS+/hPPARy
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étudiée (figure 20). Nous avons r
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PPARa PPARB Amorces sens (5' vers 3
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Taq 1 coupe uniquement le fragment
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PCR semi-quantitative Une technique
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Amorces sens Amorces anti-sens Réf
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EXPRESSION DES PPAR AU COURS DU DEV
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Figure 21 : Etude par immunocytochi
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Figure 22: Etude par immunocytochim
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Figure 23: Etude par immunocytochim
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@ 12345678 404 pb- +-G3PDH 242 pb-
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Au niveau de l'intestin, quel que s
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Enzymes Activités spécifiques 5 j
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@ Q) "0 Cti:l- .n 115 ~ ~ ~.~ CIl .
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Figure 27 : Mise en évidence de l'
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PPARa PPARy PPARy (commerciale) Fig
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même, lorsque l'anticorps primaire
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Caco-2 5 10 15 TA IR --288 -188 Fig
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COX-1 COX-1 Caco-2 Caco-2 intestin
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o ~- caténine Caco-2 !Tissu adipeu
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ARN totaux ARN Poly A+ PPARy 51015
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û) cr: ~ « .- ll.. ~ ll.. .~ .0 Q
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RT-PCR Caco-2 (jours de culture) Co
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migration électrophorétique. La q
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l'activité enzymatique de AOX et c
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RT-PCR HT29 (jours de culture) comp
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La protéine PPARy1, contribuent en
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Caco-2 ft , t. "l PPARy cytochrome
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EXPRESSION DES PPAR AU NIVEAU D'ADE
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Nombre de cas (%) SEXE Homme Femme
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Tissu sain Tumeur Témoin (grandiss
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témoin PPARy Figure 42 : Mise en
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patients 99/5984 N T 99/5027 N T 98
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ANALYSE GENERALE PPARa 2,82 0,005 S
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Paramètres PPAR Cf. PPAR ~ PPARy C
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STADES 1et 1/ PPARa 2,23 0,02 S PPA
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III. DISCUSSION a. Expression de PP
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différence significative d'express
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témoin PPARy cytochrome c Figure 4
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98/3413 98/3957 99/4514 MT (pb) il/
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dans du TBE (figure 48). Le produit
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DISCUSSION GENERALE 102
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l'expression de PPAR~ précède cel
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Il est possible d'envisager un rôl
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par conséquent difficile de relier
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même coupe. Les résultats obtenus
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1. Adams, M., M. Reginato, D. Shao,
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46. Chevalier, S., and R. A Roberts
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88. Fenoglio, C., R. Richard, and G
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132. Hirase, N., T. Kanase, Y. Mu,
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- Page 217 and 218: 148 neoplasia. STRUCTURAL ORGANIZAT
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