58Â° Congresso Nazionale SCIVAC: Oncologia veterinaria

More documents

Recommendations

Info

58° Congresso Nazionale SCIVAC • Milano, 7-9 Marzo 2008 • Oncologia veterinaria - Alle soglie del III Millennio A CASE OF COCCYGEAL CHONDROID CHORDOMA IN A CAT: CLINICAL AND HISTOPATHOLOGICAL FEATURES UN CASO DI CORDOMA DI TIPO CONDROIDE IN UN GATTO: ASPETTI CLINICI E ISTOPATOLOGICI Antonio Carminato 1 MedVet; Marta Vascellari 1 MedVet Franco Mutinelli 1 MedVet, Dipl ECVP 1 Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro (PD), Italy Introduction. Chordoma is an uncommon neoplasm arising from remnants of notochord. Chondroid chordoma has been reported in rats, minks and ferrets (Dunn et al., 1991), mostly located on the tail. Few canine and only one feline cases of chordoma have been reported (Carpenter et al., 1990). A case of feline chondroid chordoma without recurrence after surgery is described. Case report. On August 2007, a 4-year-old, intact female, shorthair cat was examined by the referring veterinarian for a 2.5 x 2 cm mass, developed beyond the last coccygeal vertebral segment 10 months before and recently fast growing. A complete physical examination excluded any further clinical alteration. Following the veterinarian’s advice, the owner decided to have the mass removed. Apical caudectomy was performed with a 2 coccygeal vertebral surgical margin. The tissue was immediately fixed in 10% neutral buffered formalin, routinely processed and paraffin embedded, for histopathological examination. Histologically, a well demarcated nodular mass involving the last vertebral coccygeal segment and the surrounding subcutaneous tissue was detected. The nodule was composed of three components concentrically arranged: lobules of vacuolated polygonal cells (physaliferous cells) at the periphery, surrounding cartilage and a central core of trabecular bone which contained marrow and haematopoietic cells. The physaliferous cells were focally surrounded by a mucinous extracellular matrix that gradually differentiated into the cartilaginous zones. Periodic acid-Schiff (PAS) staining highlighted cartilage component of the tumour besides a small quantity of intense pink granules in the cytoplasm of the vacuolated cells. Negative staining with Oil-red-O performed on frozen sections of the tumour excluded the presence of adipocytes. Immunohistochemistry was performed on 3.5 mm tissue sections for the presence of keratin (CK AE1-AE3, Dako) and vimentin (V9, Dako) intermediate filaments, and S-100 protein (Dako). Immunohistochemical staining patterns were consistent with the results of previous studies of chordoma in various species. The tumour demonstrated dual expression of keratin and vimentin intermediate filaments. In details, the lobules of physaliferous cells were intensely and diffusely positive for cytokeratin, weekly and inconstantly positive for vimentin and S-100 protein. The presence of cytokeratin 172
58° Congresso Nazionale SCIVAC • Milano, 7-9 Marzo 2008 • Oncologia veterinaria - Alle soglie del III Millennio clearly differentiated chordoma from chondrosarcoma. A diagnosis of chondroid chordoma was made. No recurrence of the tumour was present 5 months after surgery. Discussion. Chordoma is an uncommon neoplasm arising from remnants of notochord. The notochord defines the cranial-caudal axis of the embryo, induces the formation of the head and central nervous system and serves as an organizing development centre of the vertebral bodies and the basal portions of the sphenoid and occipital bones. The nucleus pulposus is believed to be the only derivative of notochordal tissue. Three distinct types are recognized in human beings: 1) the classic chordoma, 2) chondroid chordoma, and 3) chordoma with a malignant spindle cell component. The classic chordoma is a slow-growing, locally aggressive neoplasm with high rate of recurrence, particularly those of sacrococcygeal or vertebral origin, while chondroid chordoma arises primarily in the spheno-occipital region and is characterized by chondromatous and chordomatous features, showing a better prognosis compared to classic chordoma (Unni and Inwards 2000). Besides human being, chondroid chordoma has been reported in rats, minks and ferrets (Dunn et al., 1991), and is frequently located on the tail. Only one case of cervical non cartilaginous chordoma with metastasis to prescapular lymph nodes was reported in the cat (Carpenter et al., 1990). The feline case reported here shows histological characteristics similar to those of the chondroid chordoma described in ferret. It may be the greater degree of differentiation that accounts for its better prognosis when compared with classic chordoma. Only an accurate histological and immunohistochemical diagnostic approach allows a proper distinction of chondroid chordoma from other tumours of the tail with cartilaginous differentiation. References 1. Dunn DG, Harris RK, Meis JM, Sweet DE 1991 A histomorphologic and immunohistochemical study of chordoma in twenty ferrets (Mustela putorius furo). Vet Pathol 28: 467-473. 2. Carpenter JL, Stein BS, King NW Jr, Dayal YD, Moore FM 1990 Chordoma in a cat. J Am Vet Med Assoc 197: 240-242. 3. Unni KK, Inwards CY 2000 Tumors of the osteoarticular system. In Fletcher CDM Ed. Diagnostic Histopathology of Tumors, 2nd ed., pp. 1584-1585. Churchill Livingstone, London. Corresponding author: Antonio Carminato Istituto Zooprofilattico Sperimentale delle Venezie Viale dell’Università, 10 - 35020 Legnaro (PD), Italy Phone ++39 049 8084260 - Fax ++39 049 8084258 - E-mail: acarminato@izsvenezie.it 173
Page 1:
58° Congresso Nazionale SCIVAC •
Page 5 and 6:
Page 9:
Page 12 and 13:
Page 14 and 15:
nr. 6312 Sedazione IL PASSATO È FI
Page 16 and 17:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68 and 69:
Page 70 and 71:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123: 58° Congresso Nazionale SCIVAC •
Page 165 and 166: POSTER Gli estratti sono elencati i
Page 171: 58° Congresso Nazionale SCIVAC •
show all

58Â° Congresso Nazionale SCIVAC: Oncologia veterinaria

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?