Contribution à l'étude de virus de mollusques marins apparentés ...

LEGEND TO FIGURES
Figure 1 : Origin of cultured Pacifie oyster broodstocks.
Figure 2 : Survival rates of Pacifie oyster larvae held at either 22-23°C or
performed with broodstocks from four different French sites: Arcachon (
( Il ). Brest ( • ) and Marennes ( --w--).
25-26°C. Spawns were
" ). La Tremblade
Figure 3 : Growth rates of Pacifie oyster larvae held at either 22-23°C or 25-26°C. Larvae were
obtained From broodstocks originating in Arcachon • . La Tremblade O. Brest [J and Marennes • .
Figure 4 : Toluidine blue stained semi thin sections of Pacifie oyster larvae. Animais from parents
originating From Arcachon. La Tremblade and Marennes held at 22-23°C or 25-26°C exhibited nuclear
lesions. Arrows : condensed. hyperbasophilic nuclei. Arrowheads : enlarged nuclei presenting
marginalisation of chromatin.
Figures 5 to 8 : Transmission electron micrographs of herpes-like virus infected cells of Pacifie oyster
larvae held at 25-26°C. Figures 5 and 6 : Intranuclear spherical or polygonal virus particles. Some
particles appear empty (arrowheads) and other contained an electron dense core (arrows). Bar: 200
nm. Figure 7 : Enveloped viruses (arrows) within cytoplasmic vesicles in infected cell of a Pacifie oyster
larvae. Bar: 500 nm. Figure 8 : Enveloped herpes-like virus particles (arrows) found in extracellular
spaces. Bar : 500 nm.
Figure 9 : Transmission electron micrograph of nuclear alterations observed in larvae held at 22-23°C.
The nucleus appeared very electron dense. but with some electron-Iucent areas. Bar: 200 nm.
III