Contribution à l'étude de virus de mollusques marins apparentés ...

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detect a single infected cell in a microculture weil, and this improved specificity enabled a more reliable LDV titration. Acknowledgements Thanks to Dr K. Anders and Dr J. Robin for their informations on LDV and for providing LDV sampi es. This work was partially supported by the Conseil Général de la Charente Maritime. S.H. Mortensen was founded by the Royal Norvegian Council for Industrial and Scientific Research. References Anders K., 1989. Lymphacys/is disease of Fish. In Ahne, W. & Kustack, E. (Eds.), Viruses of Lower Vertebrates. Springer Verlag. 141-160. Berthiaume L., R Alain & J. Robin, 1984. Morphology and ultrastructure of Lymphocystis disease virus, a fish iridovirus, grown in tissue culture. Virology, 135 : 10-19. Comps M., 1.R. Bonami, C. Vago & A. Campi 110, 1976. Une virose de l'huître portugaise (Crassas/rea angulala Lmk). Comptes Rendus Hebdomadaires des Scéances de l'Académie des Sciences, Paris (D), 282: 1991-1993. Darai G., K. Anders, H.G. Koch, H. Delius, H. Gelderblom, C. Samalecos & RM. FIUgel, 1983. Analysis of the genome of Fish Lymphacyslis Disease Virus isolated directly from epidermal tumors of Pleuronectes. Virology, 126: 466- 479. Devauchelle G., Attias J., Monnier C., Barray S. Cerruti M., Guerillon 1. & Orange Balange N., 1985. Chilo Iridescent Virus. CUITent Topics in Microbiology and Immunology, 116: 37-48. Elston R. & M.T. Wilkinson, 1985. Pathology, management and diagnosis of oyster velar virus disease (OVVD). Aquaculture, 48 : 189-210. Essani K. & Granoff A., 1989. Amphibian and piscine iridoviruses proposai for nomenclature and taxonomy based on molecular and biological properties. Intervirology, 30 :187-193. FIUgel R.M., 1985. Lymphacys/is Disease Virus. CUITent Topics in Microbiology and Immunology, 116: 133-150. Francki R.I.B., C.M. Fauquet, D.L. Knudson & F. Brown, 1991. Classification and nomenclature of viruses. 5th Report of the International Committee on Taxonomy of Viruses. Archives of Virology, Supplementum 2, Springer Verlag. French D., E. Fischberg, S. Buhl & M.D. Scharff, 1986. The production of more usefull monoclonal antibodies. 1 : Modifications of the basic technology. Immunology Today, 7(11) : 344-346. Lorenzen K. & P.F. Dixon, 1991. Prevalence of antibodies to Iymphacyslis virus in estuarine flounder Pla/ichtys f1esus. Disease of Aquatic Organisms, Il : 99- 103. Midlige F.H. & RG. Malsberger, 1968. In vitra morphology and maturation of Lymphacyslis virus. Journal ofVirology, 2(8) : 830-835. Peeples M.E., 1987. Differentiai detergent treatment allows immunofluorescent localisation of the newcastle virus matrix protein within the nucleus of infected cells. Virology, 162 : 255-259. 195
Reed 1.L. & H. Muench, 1938. A simple method of estimating fifty percent endpoints. American Journal of Hygiene, 27 : 493-497. Robin 1. & L. Berthiaume, 1981. Purification of Lymphocystis disease virus (LDV) grown in tissue culture. Evidences for the presence of two types of viral particles. Revue Canadienne de Biologie, 40(4) : 323-329. Robin J., C. Larivière-Durand & J. Bernard, 1983. The chemical composition of Lymphocystis Disease Virus of fish. Revue Canadienne de Biologie Experimentale, 42 : 173-176. Russel P.H., 1974. Lymphocystis in wild plaice Pleuronectes platessa (L.), and flounder, Platichtys flesus (L.), in British coastal waters: histopathological and serological study. Journal ofFish Biology, 6: 771-778. Schnitzler P., A. Rosen-Wolff & G. Darai, 1990. Molecular biology of Fish Lymphocystis Disease Virus. Molecular Biology ofiridoviruses, Ed. G. Darai, chap. JO : 203-234. Samalecos C., 1986. Biochemical and structural studies of fish Lymphocystis disease virions isolated from skin tumors of Pleuronectes. Journal of Virological Methods, 13(3) : 197-205. Stitz L., H. Hengartner, A. Althage & R.M. Zinkernagel, 1987. An easy and rapid method to screen large numbers of antibodies against internai cellular determinants. Journal of Immunological Methods, 106 : 216-221. Legend to figures Figure 1 : A native red fluorescence on healthy BF2 cells is observed by indirect immunofluorescent assay using monoclonal antibodies specific for LDV. Figure 2 : Indirect immunofluorescent assay using 15DIIC9 monoclonal antibody on LDV infected BF2 cells reveal cytoplasmic tiny dots two days post infection (p.i.)(2a), which became larger dots on the seventh day p.i. (2b). A green fluorescence throughout the cytoplasm was observed on the ninth day p.i. (2c). 196
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1 1 1 1 Année 1995 UNIVERSITE DE B
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à mes parents à Stéphane
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1 1 ) TABLE DES MATIERES INTRODUCTI
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3 - Essais d'infection expérimenta
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INTRODUCTION
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d'approche pour contrôler les mala
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1 1 1 1 1 1 1 1 j Un premier chapit
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DONNEES BIBLIOGRAPHIQUES 1 - Rappel
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1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
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et Wray, 1952 ; Mackin, 1962 ; Burr
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1 1 1 1 1 1 1 1 des zones indemnes
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3 - Procaryotes Les mollusques biva
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Tableau 4 : Infections à Chlamydie
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cheptels de C. angulala, a conduit
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III - Caractéristiques principales
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1 1 1 1 1 1 1 1 1 1 1 J 3.2 - Entr
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1 1 1 1 1 1 1 1 1 1 1 PREAMBULE Pub
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Chapitre 1 : Caractérisation d'un
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l 1 1 1 1 1 1 1 1 1 1 1 1 PUBLICATI
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References AllIle . w .. SchlOl fc
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Platichtys flesus, plie, Pleuronect
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Figure 1 : Marquage de coupes de do
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CHAPITRE 2 Comparaison antigénique
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Ces électrophorèses ont égalemen
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1\6 , HMW LMW Figure 1 : Caractéri
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a 2 3 4 5 6 7 8 9 10 --- - - - - Il
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Tableau 2 : Comparaison anti géniq
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Figure 4 : Coupe histologique d'hû
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Chapitre 1 : Mise en évidence et d
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Les virions observés chez les larv
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• •• Figure 2 : Cliché en mi
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2 - Participation à la mise en év
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Figure 10 : Lésions histologiques
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Bu ll. Eur. Ass. Fi sh Palhal.. 14(
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736 Material and methods Samples of
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740 RENAULT (T.) ET COLLABORATEURS
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742 15. - MURP HY (F.A.) and K INGS
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Chapitre Il : Essais de reproductio
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2 - Essais d'infection expérimenta
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CHAPITRE 3 Effet de la température
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26°C), de détecter la présence d
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présentent des lésions histologiq
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Introduction The Pacific oyster, 'C
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(Table 1). 100% mortality in this g
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than at high temperature (25-26°C)
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Our plans for testing this la st hy
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LEGEND TO FIGURES Figure 1 : Origin
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CHAPITRE IV : Essais de propagation
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Figure 1 : Principales caractérist
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2 - Essais d'infection de primocult
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(SVF) et/ou d'hémolymphe de Crassa
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68 0.2 g/I, KCI, No. P 1300' 2.77 g
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72 2. Cousserans F, Bonami lR, Comp
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2.2. Essais d'infection de primocul
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Chapitre V : Mise au point d'un pro
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Du fait de la présence d'une coqui
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• " (1 - " 100nm 100nm Figures 1
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Chapitre VI : Mise au point de mét
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Page 175 and 176: CONCLUSION L'ensemble de ce travail
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Page 179 and 180: REFERENCES BIBLIOGRAPHIQUES Ahne W.
Page 181 and 182: Cahour A., 1979. Mar/eilia refringe
Page 183 and 184: Darlington R.W. et Moss L.H., 1969.
Page 185 and 186: Girard M. et Hirth L., 1989b. Méth
Page 187 and 188: Johnson D.C. et Spear P.G., 1982. M
Page 189 and 190: Lemaster S. et Roizman B., 1980. He
Page 191 and 192: Ohba M., Kanda K. et Aizawa K., 199
Page 193 and 194: Read G.S. et Frenkel N., 1978. Herp
Page 195: Shuster C.L. et Hillman T.E., 1963.
Page 200 and 201: 1994 : P. Maffart, R.M. Le Deuff, T
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