iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
Event Modules
Event
Options under the Event menu allow you to specify events, create Phantom
events, and create Associations.
The Event module creates events that are defined by a threshold contour. You can
set up the integration peripheral and background contours.
Contour Tab
Selecting the Contour tab from the Event menu opens the following menu.
From the Contour tab, you can set up the integration peripheral and background
contours.
Integration contour—Defines the boundary within which the total fluorescence
of the event will be calculated (integrated).
Expand by—Specifies the number of pixels to generate the integration
contour outside the threshold contour. That is, if you specify Expand by 3, the
integration contour is drawn 3 pixels outside the threshold contour.
Color—Select the color of the peripheral contour line. If color is set to None,
no visible contour will be drawn.
Show event id—Displays the event ID for each event in the scan image.
Background Tab
Selecting the Background tab opens the following menu.
Use Dynamic Background—If checked, the system uses two boundary lines to
define an average background value that is subtracted from the fluorescence based
features for each contoured event.
Distance—Specifies the distance between the integration contour and the first
background contour. Set the first background contour away from the
integration contour such that it is away from the event fluorescence.
3-56 iCys Research Imaging Cytometer User Guide Revision B