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iCys User Guide

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Using the iNovator Toolkit iNovator-specific Protocol Modules<br />

Whole section<br />

dynamic scan area<br />

Pictured below, on the same Tissue Micro-Array element, are the three types of<br />

dynamically generated scan areas (outlined in yellow). The numbers shown are the<br />

event IDs generated by the Show event name option.<br />

Multi-Scale Association<br />

Array Alignment<br />

Rectangular dynamic scan area<br />

—placed at centroid of element<br />

Random dynamic scan area<br />

—three scan areas specified<br />

The Multi-Scale Association module is analogous to the Association module as<br />

described on page 3-58 except that it operates across first and second scale scans<br />

whereas the Association module operates within first or second scale scans (or single<br />

scale scans.) This is illustrated in Tutorial Exercise 4 where the second scale event<br />

component is associated with the first scale Tissue component. There are no<br />

parameters for the Multi-Scale Association module.<br />

The Array Alignment module is typically used when doing a low resolution scout scan<br />

to identify the elements of a Tissue Micro-Array. The module identifies the contoured<br />

events for the component to which it is attached, as an ordered pair of numbers. This<br />

ordered pair represents the Column and Row for the element within the larger array.<br />

This is pictured below.<br />

B-26 <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> Revision B

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