iCys User Guide

iCys Research Imaging Cytometer Hardware Reference Microscope Unit
Microscope Unit
The microscope unit consists of the following:
The Microscope
❑ A standard Olympus IX-71 microscope.
❑ WH10X eyepieces.
❑ An adapted motorized stage – the manual stage positioning controls, located in
front of the microscope, allow for coarse or fine operator control of the motorized
stage positioning.
❑ A brightfield illumination column and lamp housing and TH4-100 power supply
for brightfield lamp.
❑ An optional EpiFluorescence (Reflected fluorescence) system, including a power
supply for the 100W mercury burner.
❑ Sony CCD Video Camera.
❑ A forward scatter detector located above the microscope stage.
❑ The scanning laser beam is directed into the microscope through a side port on the
microscope. This beam is directed to the microscope objective and focused on the
sample located on the microscope stage. Fluorescent light, collected by the
microscope objective, emerges through this port and is relayed to the
photomultiplier tubes located in the optics/electronics unit.
❑ Other optional microscope accessories may also be included.
The microscope supplied with iCys is an Olympus IX-71 which comes with an
Olympus objective turret containing 10X, 20X, and 40X Universal Plan Semi-
Apochromatic objectives. It also contains a crosshair alignment tool.
Note: Do not remove, replace, or add objectives to the microscope unit.
iCys can be purchased with or without the AutoFocus feature. The microscope
functions are directly affected by the presence of AutoFocus, as follows:
❑ If your iCys system does not have the AutoFocus feature, you will need to
manually move the objective lens into the correct positions as discussed in “Using
the Microscope without AutoFocus” on page 2-8.
❑ If your system does have AutoFocus, see “Objective Lens Automated Movement”
on page 2-9.
Using the Microscope without AutoFocus
If your iCys system does not have the optional AutoFocus feature, then you will need
to manually focus the microscope on the specimen in the area to be used during the
run before starting a Test Scan or Scan & Save run.
You can manually focus by either:
2-8 iCys Research Imaging Cytometer User Guide Revision B