iCys User Guide

Administrator Tasks Forward Scatter Pixel Correction
Forward Scatter Pixel Correction
When images are collected using the forward scatter mechanism, variations in pixel
intensity are produced because the laser beam strikes the sensor at various angles
during its “sweep.” In order to produce the best quality images, these variations must
be corrected.
The Administration menu offers a Forward Scatter Pixel Correction utility. The
opening screen is shown below:
The textbox on the left contains instructions on how to proceed. List boxes allow
selection of a laser and objective. Each combination of laser and objective must be
corrected.
Once selections are made, press the Measure button. The utility will move the empty
stage to the center and automatically adjust the gain and offset for the best exposure.
The textbox on the left will display the steps of the measurement.
The minimum and maximum values of pixel brightness required to pass these
adjustments are stored in the calibration file under “scatter-auto-exposure.” The
factory settings should work in most circumstances.
As the adjustments progress, you can see the results in the image window. When the
adjustments are complete, the textbox will display the message “Scan complete.”
When the measurement is complete, a message box will display the message
“Measurement complete.”
After clicking OK on the message box, you will note that the Save button has become
available. You may elect to save the correction factors now or wait until you make
another measurement. When the Measure button is pressed again, the current
correction data will be replaced with new data.
C-26 iCys Research Imaging Cytometer User Guide Revision B