iCys User Guide

iCys Research Imaging Cytometer Hardware Reference iCys Hardware Setup
6. For viewing, if the camera is on (as described in “Observing Samples With the
Video Camera” on page 2-16) you can select the viewing mode by sliding the
Light Path Selector knob as shown in Figure 2-10, “Light Path Selector Knob,” on
page 2-15.
7. Focus the sample using the microscope focus knob or, if available, the rocker
switch and the fine focus knob on the Remote Control unit.
8. After the hardware setup is complete, you can use the iCys application to view the
samples on the microscope stage.
Note: When using EpiFluorescence, you may cause photobleaching in the
exposed samples. Photobleaching is caused by exposing fluorophores to
light which, over time, destroys the fluorophores irreversibly and results
in loss of fluorescence. The degree of photobleaching increases with the
time and intensity of light exposure.
When using the EpiFluoresence feature prior to scanning, minimize
sample exposure and when possible scan in areas that have not been
exposed to EpiFluorescence illumination.
For detailed information about the EpiFluorescence system, see the Olympus
Instructions: Manual/Motorized Reflected Fluorescence System.
Observing Samples With the Video Camera
iCys comes with a 3CCD camera module, EPIX PIXCI ® Imaging Board, and XCAP-
Lite image viewing software. You can use the camera while observing to magnify and
save images from the computer monitor.
➣ To use the video camera:
1. Turn on the color camera monitor.
2. From your PC, click on XCAP for Windows to open the EPIX XCAP-Lite
application.
3. When iCys scans a sample or is moved from one event to another, the visual
output is displayed on the camera monitor, if on, and on your PC, if XCAP-Lite is
open.
From XCAP-Lite, you can capture and save screenshots of desired images. See the
online help for XCAP-Lite for more information.
2-16 iCys Research Imaging Cytometer User Guide Revision B