iCys User Guide

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Using the iNovator Toolkit Multi-scale Scanning Analyze events (bypass-bypass) Select this option if you do not want to rescan any existing images or acquire new data, but want to work only with the existing event data from a previous scan. Reanalyze scale 1 (virtual-bypass) Select this option if you want to virtually rescan existing scale 1 data, that is, reprocess scale 1 image data after modifying the protocol. Modify the protocol to optimize the analysis, then click Scan and Save to generate new event data and DSAs and save the data as a new run. Reanalyze scale 1 and scan scale 2 (virtual-live) This option allows you to reprocess scale 1 image data as described above and then to acquire new data from a live scale 2 scan of your sample. Modify the scale 1 protocol to optimize the analysis, then click Scan and Save to generate new scale 1 event data and DSAs and save the data as a new run. To continue with the 2nd scale scan, manipulate the DSAs if desired, and then click the Paused box on the bridge between the Scan modules to initiate the 2nd scale scan. Reanalyze scale 2 (bypass-virtual) Select this option if you want to use the saved 1st scale scan "as is" and virtually reanalyze the already acquired 2nd scale scan data. To initiate the 2nd scale scan, click Scan and Save. B-36 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Using the iNovator Toolkit Note that the Mode signature (such as virtual-bypass) serves as shorthand for the type of run that is being performed. This mode type is shown in the Scan Module graphics, as shown below. Revision B iCys Research Imaging Cytometer User Guide B-37
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User Guide DOC190-0037-001 Revision
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Contents Chapter 1 Introduction Man
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May 6, 2005 Contents iCys Status Ba
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May 6, 2005 Contents Appendix B Usi
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Chapter 1 Introduction This manual
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May 6, 2005 Introduction System Ove
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Chapter 2 iCys Research Imaging Cyt
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May 6, 2005 iCys Research Imaging C
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Chapter 3 iCys Cytometric Analysis
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May 6, 2005 iCys Cytometric Analysi
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Chapter 4 Generating Analytical Dat
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May 6, 2005 Generating Analytical D
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Page 197 and 198: Appendix A Installing iCys Research
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Page 203 and 204: Appendix B Using the iNovator Toolk
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