iCys User Guide

iCys Research Imaging Cytometer Hardware Reference iCys Hardware Setup
Scanning Samples with iCys
➣ To scan samples with iCys, you need to make sure that the microscope is set as follows:
1. Set the light path selector lever to side port ( ).
Note: When set to observation ( ), scanning will not take place.
2. Set the magnification selector knob to 1X magnification (the pushed in position).
3. If your system has the optional EpiFluorescence feature, set the EpiFluorescence
cassette to position 1.
4. Set the condenser filter turret to position 5.
Note: Position 5 of the condenser filter turret is used for brightfield and Forward
Scatter and must be empty.
5. Make sure the condenser is rotated in the down position (not rotated upwards).
6. After the hardware setup is complete, you can use the iCys application to set up
and scan as described in Chapter 3, “iCys Cytometric Analysis Software
Application Overview.”
Observing Samples with the Brightfield Source
➣ To observe samples using the brightfield source:
1. Set the light path selector lever to observation ( ).
2. Turn on the following:
❍ The TH4-100 Power Supply (shown in Figure 2-6, “Brightfield Illumination
Assembly,” on page 2-10).
❍ The brightfield ON/OFF switch (shown in Figure 2-9, “iCys Microscope
Selected Components,” on page 2-13).
You may want to set the Light Control Intensity knob on the TH4-100 to “Max”
and use the Light Intensity Control knob on the main instrument to control the
brightfield illumination.
3. Select Brightfield from the iCys Instrument menu to open the brightfield shutter.
For more information, see “Instrument Menu” on page 3-6.
4. If your system has the optional EpiFluoresence feature, set the EpiFluorescence
cassette to position 1.
5. For viewing, if the camera is on (as described in “Observing Samples With the
Video Camera” on page 2-16) you can select the viewing mode by sliding the
Light Path Selector knob as shown in Figure 2-10:
2-14 iCys Research Imaging Cytometer User Guide Revision B