iCys User Guide

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iCys Cytometric Analysis Software Application Overview iCys Task Bar Scan and Save Make sure that none of the dimmest pixels are reading 0 and the background pixel values are consistently in the range of 300 to 500. Note: Offset range is between -5 and 5 volts. Changing offset to correctly adjust background pixels will also affect bright pixels. Check bright pixels after offset adjustments, and adjust voltage if necessary. Note: Staining often varies across the specimen; review multiple scan fields to ensure that settings are appropriate. 9. When you are satisfied with the settings, you can confirm that they are optimized by using the bright contrast tool in the field image window. Once you are satisfied with the settings for your scan, you can click the Scan and Save button. Scan and Save begins the formal scan process. ➣ To Start a Scan and Save: 1. Click Scan and Save from the Task Bar. The Scan and Save dialog displays: 2. Enter a Run name. 3. Enter a Run Annotation if desired 3-84 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 iCys Cytometric Analysis Software Application Overview Instrument Section 4. Check appropriate Data Saving entries. Save images as either Jpeg or Flat. 5. Click OK. The window closes and the iCys scan begins. Keep in mind that during Scan and Save, most options are disabled. When you stop the scan before all scan areas have been scanned, you will be given the chance to keep the data that has been already saved: If you select No, the all run data already saved will be deleted. The run number will not be reused, so the number for the next saved run will be incremented. Laser Shutter buttons The laser shutter buttons allow you to manually open laser shutters. When the laser shutter is open, the button appears pressed and the laser graphic is colored. During scan the buttons update to reflect the current shutter status. Objective pulldown Use the Objective pulldown to let the software know which objective is currently selected in the turret. Revision B iCys Research Imaging Cytometer User Guide 3-85
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User Guide DOC190-0037-001 Revision
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Contents Chapter 1 Introduction Man
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May 6, 2005 Contents iCys Status Ba
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May 6, 2005 Contents Appendix B Usi
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Chapter 1 Introduction This manual
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May 6, 2005 Introduction System Ove
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Chapter 2 iCys Research Imaging Cyt
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Chapter 3 iCys Cytometric Analysis
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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May 6, 2005 Using the iNovator Tool
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Appendix C Administrator Tasks This
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Appendix D Glossary This chapter co
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iCys User Guide

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