iCys User Guide

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iCys Cytometric Analysis Software Application Overview iCys Task Bar Region Image Refresh Scan Section Test Scan Table 3-7: Window Icons Specific to the Well Image Option Icon Meaning Show Label Selecting Show Label overlays a grid that represents the boundaries of the constituent field images that have been assembled to form the well image. Create Region This feature is available only with the iNovator Toolkit. Displays an image of the region being scanned. This feature is available only with iNovator Toolkit. Updates and redraws all images. Use the Scan section of the task bar to run either a Test Scan or a full Scan & Save operation. This section discusses the following topics: ❑ Test Scan, page 3-80 ❑ Scan and Save, page 3-84 Performing a Test Scan enables you to adjust all of your iCys settings before executing a Scan & Save operation. Test Scan performs a scan, which allows you to see and adjust your PMT sensors (and other settings) but does not save any data from the scan. 3-80 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 iCys Cytometric Analysis Software Application Overview Additionally the Test Scan operation allows you to analyze the data and define regions in scattergrams and histograms before you perform a Scan & Save. The six buttons at the top of the Scan section of the task bar control the Test Scan function. These are described in the table below. Table 3-8: Start/Pause/Resume Single Field or Next Scan During a Test Scan the Scan Area Setup window displays the scan status of the wells or scan areas. However, you cannot select any of the icons from the toolbar. Additionally, the following options are disabled from the task bar: ❑ New Run ❑ Open Run ❑ Load ❑ Statistics ❑ Refresh Revision B iCys Research Imaging Cytometer User Guide 3-81 , Use this button to: ❑ Start a Test Scan ❑ Pause a scan that has been started ❑ Resume a scan that has been paused. Acquires first field and pauses, or advances to and scans the next scan field. Next well Advances to and scans the next well Rescan Rescans current field Previous field Returns to and scans previous field Scan Only Active Wells If selected, iCys scans only those scan areas selected in the Carrier window. For more information about the Carrier window, see “Setting up a Microplate Scan” on page 3-17 and “Setting up a Freeform Carrier Scan” on page 3-22. Stop scan Stops the current scan
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Contents Chapter 1 Introduction Man
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Chapter 1 Introduction This manual
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Chapter 2 iCys Research Imaging Cyt
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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Appendix D Glossary This chapter co
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iCys User Guide

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