iCys User Guide

May 6, 2005 iCys Cytometric Analysis Software Application Overview
❑ Channel—If you have chosen Background, Integral, or MaxPixel, the Channel
field will be populated with the channels in this protocol. You must select which
channel to use for analysis.
❑ Region—If you have created any regions on a scattergram or histogram display,
you can choose to create statistics based on any of these regions. If no regions
have been created, this field will remain blank.
Note that regions are specific to event components. That is, if a Region 2 is
defined in a scattergram for Primary events it will be available for statistics for
Primary events but not for other components.
❑ Label—If the Use Default box is checked in the Label field, the Statistic will be
automatically labeled according to the parameters selected. Unchecking the box
allows you to enter your own label.
❑ Scale Min Max—These values determine the scale used when showing
expression levels in the Microplate window (see “Showing Expression Levels” on
page 3-20).
Note that the statistics are calculated only for components that are lower in the
association hierarchy than the component (or well) tab. For example in the example
below the component hierarchy (established using the Association2 module) is:
❍ Well
✧ Primary
✧ Sub1
✧ Sub2
Label field
Revision B iCys Research Imaging Cytometer User Guide 3-65