iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
( ) to check the focus. Keep changing the focus and refreshing the scan
until you are happy with the results.
The displayed value on the bottom of the Field Image window (AF Offset:)
changes as you adjust the focus knob.
6. When the scan image appears as desired, click the Set Focus Offset button to
set the current value of the User Offset.
The new User Offset is used for all scans in the current Workspace. However,
the new value is not saved to the Workspace file until the Workspace is saved.
Note: If the scan completes before you click the Set Focus Offset button, the
offset returns to zero, the Set Focus Offset is deactivated, and the Adjust
Focus Offset button is cleared. Additionally, the focus knob is disabled
while scanning.
Saving a User Focus Offset
After the User Offset has been set, click on Save Workspace to preserve the new
value in the Workspace File. If you exit iCys without saving the Workspace file,
the new offset will be lost, and the next time you load that Workspace file the
previous value will be loaded.
The Scan View Status Bar
The Status bar is at the bottom of the Scan View window. It provides the following
information:
Table 3-6: Scan View Status Bar
Status Detail Meaning
Cursor position The XY coordinates of the cursor position either in
microns or pixels.
Pixel value The intensity of the pixel over which the cursor is
hovering. Values can range from 0 to 16383.
Scan number The number of the current scan.
Zoom value The magnification factor as set by the Zoom in and Zoom
out buttons.
Grid Size Displays the Grid size in pixels
AutoFocus offset If applicable, the offset established using the Adjust focus
offset and Set focus offset buttons.
3-70 iCys Research Imaging Cytometer User Guide Revision B