iCys User Guide

May 6, 2005 Using the iNovator Toolkit
scan lines wide, and so on. A square scan field is 768 scan lines wide (because
the Y dimension is always 768 pixels tall.)
❍ Scan field dimension—Displays the size of each pixel based on the
parameters you have selected.
❑ Advanced Tab—When the Advanced tab is selected the following options
appear.
❍ Scan mode—Describes the current scan mode (Virtual, Live, Bypass)
✧ Live—In this mode the Mosaic Scan acquires new data by scanning the
sample.
✧ Virtual—In this mode the Mosaic Scan re-contours the saved data.
✧ Bypass—In this mode the Mosaic Scan in the first scale is by-passed and
the user may perform a virtual second scale scan.
For more information on scan modes see the section entitled “Analysis Modes
for Multi-scale Scans” on page B-34.
❍ Correct Pixels—Checking this option enables a correction that accounts for
variations in collection efficiency along the scan line. This is performed on
each of the images as they are acquired. See also “Correct Pixels” on
page 3-50.
❍ Sampling mode—When generating the Mosaic images, iCys combines pixels
from the individual Scan Fields to generate the 1:1 aspect ratio needed in the
Mosaic image. There are three selections for the algorithm that iCys may use
to calculate the resultant pixel value in the Mosaic image. These are:
✧ Single—iCys uses the first pixel in the sampling bin.
✧ Average—iCys uses the average value of the pixels in the sampling bin.
✧ MaxPixel—iCys uses the pixel in the sampling bin with the maximum
value.
Revision B iCys Research Imaging Cytometer User Guide B-9