iCys User Guide

May 6, 2005 Using the iNovator Toolkit
Once the protocol parameters have been set, select Scan and Save and the iCys will
scan the 1st scale, automatically identify Dynamic Scan Areas and then scan these
areas with the 2nd scale protocol.
Live (Scout only) and Live-Pause-Live
To scan only the first scale and then either save the data for later action or manipulate
the Dynamic Scan Areas prior to continuing with the 2nd scale scan, set the bridge
between the two Scan modules to Pause by clicking on the box on the bridge until
Pause appears, as shown in the figure below.
When the first scale scan is complete, the Pause box will turn pink and Pause will turn
to Paused:
In this paused state, you may review the Dynamic Scan Areas in the Well Image
window. You have two possible options at this point:
❑ Save the scout scan data for a later scanning of the 2nd scale scan,
❑ Continue with the 2nd scale scan now.
To save the scout scan data for later, click Stop and then yes to save the run. We will
discuss opening and continuing with scout-only runs in the next section.
If you intend to continue with the 2nd scale scan, you may first review the Dynamic
Scan Areas and if necessary, move or delete them, or create new ones. For more
information on this process, see “Manipulating Dynamic Scan Areas” on page B-31.
Once you are happy with the DSAs, you may resume scanning by clicking on the
bridge; the box will become clear and scanning will resume.
Reanalyzing Existing Runs
When the user selects Open Run, several options are presented in the Analysis Mode
area of the dialog box highlighted below.
Revision B iCys Research Imaging Cytometer User Guide B-35