iCys User Guide

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Generating Analytical Data Contours ➣ To add peripheral contours: 1. Check the Peripheral contour box. Peripheral contouring will be inactive unless this box is checked. 2. In the Distance field, enter the number of pixels between the threshold contour and the inner annulus of the Peripheral contour. 3. Enter the width in pixels of the Peripheral contour in the Width field. 4. Select a color for the Peripheral contour. 4-10 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Generating Analytical Data When you use Peripheral contouring (yellow in the above example), the integral value generated is expressed as the Peripheral integral feature. It is independent of the Integral contour, expressed as the Integral feature. MaxPixel data can also be measured in the Peripheral contour, and is expressed as the Peripheral Max feature. Phantom Contours Phantom contours are artificial events that are generated by the iCys software from circular contours that are applied over the laser-scanned image. These phantom contour events have the following features: ❑ Integral ❑ MaxPixel ❑ X Position ❑ Y Position ❑ Area ❑ Time ❑ Scan Phantom contours may be laid down within a Field Image in a random fashion or in a lattice (two-dimensional array.) They are typically used in applications where you want to assess and quantify the fluorescence expression of the image, independent of the actual events. Revision B iCys Research Imaging Cytometer User Guide 4-11
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User Guide DOC190-0037-001 Revision
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Contents Chapter 1 Introduction Man
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May 6, 2005 Contents iCys Status Ba
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Chapter 1 Introduction This manual
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Chapter 2 iCys Research Imaging Cyt
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