iCys User Guide

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Generating Analytical Data Creating and Changing Scattergrams After creation, the quadrants can be dragged to any position. When data is scanned, the percentage of data in each quadrant will appear in the graph. ❑ 2-Parameter Histogram—2-parameter histograms, provide a method displaying a third (Z) parameter in the scattergram using color to represent the Z axis. If you select 2-Parameter Histogram, you can choose one of the following options: ❍ Density—Color codes the data according to how many events have been assigned to a given pixel. ❍ Expression—Color codes the data according to the value of the expression you choose in the accompanying pulldown menus. 2-parameter histograms are described in greater detail in “About 2-Parameter Histograms,” below. 4-28 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Generating Analytical Data About 2-Parameter Histograms 2-parameter histograms display aggregated data based on two parameters. iCys uses a color scale to show the value of a particular bin. A 2-parameter histogram displays in iCys, as shown below. iCys generates a 2-parameter histogram file for every scan area of every scattergram for which expression or density histograms have been requested. 2-parameter histogram files can also be viewed from iBrowser. ➣ To generate 2-parameter histogram, check 2-Parameter Histogram. You can display your data in color in one of two ways, Density or Expression: ❑ Density—Color codes the data according to how many events have been assigned to a given pixel. The more events accumulated, the higher the color of that pixel will be on the scale. This allows you to visually assess the distribution of the data. ❑ Expression—Color codes the data according to the value of the expression you choose in the accompanying pulldown menus. You may choose, for example, Integral of Green fluorescence as an expression on a graph of scatter vs. long red fluorescence. Although the graph does not display green fluorescence as a parameter, those events with the most green fluorescence will show as a higher color on the scale. For more information about 2-parameter histograms in iBrowser, see the iBrowser Data Integration Software User’s Guide. Revision B iCys Research Imaging Cytometer User Guide 4-29
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Contents Chapter 1 Introduction Man
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May 6, 2005 Contents Appendix B Usi
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Chapter 1 Introduction This manual
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iCys User Guide

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