iCys User Guide

May 6, 2005 Generating Analytical Data
About 2-Parameter Histograms
2-parameter histograms display aggregated data based on two parameters. iCys uses a
color scale to show the value of a particular bin.
A 2-parameter histogram displays in iCys, as shown below.
iCys generates a 2-parameter histogram file for every scan area of every scattergram
for which expression or density histograms have been requested. 2-parameter
histogram files can also be viewed from iBrowser.
➣ To generate 2-parameter histogram, check 2-Parameter Histogram. You can display
your data in color in one of two ways, Density or Expression:
❑ Density—Color codes the data according to how many events have been assigned
to a given pixel. The more events accumulated, the higher the color of that pixel
will be on the scale. This allows you to visually assess the distribution of the data.
❑ Expression—Color codes the data according to the value of the expression you
choose in the accompanying pulldown menus. You may choose, for example,
Integral of Green fluorescence as an expression on a graph of scatter vs. long red
fluorescence. Although the graph does not display green fluorescence as a
parameter, those events with the most green fluorescence will show as a higher
color on the scale.
For more information about 2-parameter histograms in iBrowser, see the iBrowser
Data Integration Software User’s Guide.
Revision B iCys Research Imaging Cytometer User Guide 4-29