iCys User Guide

May 6, 2005 iCys Cytometric Analysis Software Application Overview
❍ In the Scan Cycle Time field, enter the minimum number of seconds between
subsequent scan start times. If the scan finishes before the cycle time has
elapsed, iCys will wait until the next cycle to start scanning again. This allows
you to, for example, record snapshots at specific intervals of time.
Focus and Performance
Clicking the Focus and Performance icon ( ) opens the following menu.
The Focus and Performance menu functions are described below.
Focus
Allows you to select whether you want to focus the microscope manually or allow
iCys to AutoFocus for each scan area:
❍ AutoFocus—If your system uses the optional AutoFocus feature, a
✧ Focus Method—Specify the preferred focus method.
✧ Bottom then Top—For thicker plastic-bottomed plates (greater than
400µ) especially; iCysiCys will focus first on the plate bottom, then
translate up by the bottom thickness to the plate top and refocus.
✧ Bottom Only—For glass-bottomed or thinner plastic-bottomed (less
than 400µ) plates, iCys focuses directly on the bottom then moves up
by the bottom thickness plus other offsets to bring the sample into
focus.
If the carrier type supports only one focus method, the supported method
will be selected and the unsupported method will be grayed out and
unavailable.
Revision B iCys Research Imaging Cytometer User Guide 3-33