iCys User Guide

May 6, 2005 Generating Analytical Data
Table 4-2: Scattergram and Histogram Feature Parameters (Continued)
Feature Meaning
Cycle For repetitive scans, the cycle number within the
series of scans.
Integral Integrated fluorescence per event for each saved
channel.
Integral (Sub *) For primary events if there are subcontours—The
Integrated fluorescence per sub event for each saved
channel.
MaxPixel Brightest pixel value per event for each saved
channel.
Parent ID Subcontours only—The WellID of the primary event
to which this subcontour is related.
Perimeter The number of microns that comprise the perimeter
of the event.
Peripheral Integral Peripheral contours only —The integral of the
fluorescence in the area defined by the peripheral
contour.
Peripheral Max Peripheral contours only —The MaxPixel in the area
defined by the peripheral contour.
Scan Pixel number, from 0 to 768, indicating the yposition
of the scanning mirror where the event was
detected.
Time Time, in seconds, at which event was acquired.
When rescanning a Raw file, the Time parameter
contains the Well Number of this scan area.
Well # Well number (or scan area number) of event.
X position X position in the carrier for each event.
Y position Y position in the carrier for each event.
Z position Z position in the carrier for each event.
Tip: You can combine parameters by choosing a numerator as well as a
denominator parameter. The resulting parameter will reflect the value of
the numerator divided by that of the denominator.
❑ Channel —Assign the channel to display for features that are channel-specific.
❑ Log scale—If checked, displays this parameter in a base 10 logarithmic scale
(that is 0= 10 0 , 1= 10 1 , and so on). This can be useful if the data for this parameter
has a very broad range. For example, integral data may display better in log scale.
Revision B iCys Research Imaging Cytometer User Guide 4-25