iCys User Guide

May 6, 2005 Administrator Tasks
❍ A well at or near the center top row
❍ A well at or near the center bottom row
❍ A well at or near the center left column
❍ A well at or near the center right column
For example:
5. Determine which of these 9 positions yields the lowest value, and enter that value
as the Base To Well Height.
Determining the Bottom Thickness
➣ To determine the bottom thickness:
1. Move the stage so that the objective focuses on the well with the lowest Base to
Well Height (which you determined in “Finding the Base to Well Height” on
page C-14), and click on Move To from the Base to Well Height field.
2. Make sure the well you use for focusing is empty.
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3. Slowly rotate the fine focus knob counter-clockwise until the second line or lines
appear to be finely focused on the autofocus monitor. For thicker-bottomed plates,
this may take 5 or more knob rotations.Click Set from Z for the Bottom
Thickness field of the Z tab.
Revision B iCys Research Imaging Cytometer User Guide C-15
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