iCys User Guide

iCys Research Imaging Cytometer Hardware Reference Microscope Unit
Brightfield Lamp
The brightfield illumination column and lamp allow you to view your samples directly
from the microscope stage. Brightfield is only useful when using direct observation.
Figure 2-6: Brightfield Illumination Assembly
When using the brightfield lamp, you also need the TH4-100 power supply that
powers the lamp.
For information about using the brightfield lamp, see “Observing Samples with the
Brightfield Source” on page 2-14.
For detailed information about the IX71 microscope and brightfield illumination, see
the Olympus Instructions: IX71/IX51 Inverted Research Microscope/Inverted Basic
Microscope.
Forward Scatter Detector
Brightfield
Shutter
Brightfield
Filter Housing
Brightfield
Lamp
Brightfield
Power Source
Light Intensity
Control Knob
Power Switch
The Forward Scatter detector allows you to generate scatter images in iCys. You can
use scatter detection to show relief details in an otherwise flat image. iCys provides
two types of scatter imaging: Light Loss and Shaded Relief. Scatter detection is only
available when the Scatter channel is selected in the Instrument Setup window.
For information about using the Scatter detector, see “Selecting the Forward Scatter
Method” on page 3-48”.
2-10 iCys Research Imaging Cytometer User Guide Revision B