iCys User Guide

Generating Analytical Data Creating and Changing Scattergrams
Creating and Changing Scattergrams
This section contains the following topics:
❑ About Scattergrams, page 4-22
❑ Using the Graph Property Window, page 4-23
❑ Setting Scattergram Regions, page 4-30
❑ Setting Region Attributes, page 4-31
❑ Viewing Cell Details, page 4-35
❑ Other Scattergram Information, page 4-37
About Scattergrams
Scattergrams are two parameter graphs that allow you to view and save a wide variety
of data during an iCys scan. Each spot on a scattergram represents an event that
corresponds to the attributes you have selected.
Within an iCys scattergram, you can create one or more regions. A region is a subset,
or area of interest, within the scattergram from which you can:
❑ Create relationships between scattergrams and histograms so that the data in one
graph is derived from events that are within regions in other graphs. This is called
“gating,” and is described in greater detail in “Scattergram and Histogram Event
Type Tab” on page 4-26.
❑ Generate statistical analyses of the events in regions. For more information, see
“Viewing Cell Details” on page 4-35 and “Displaying Region Statistics” on
page 4-18.
❑ Add color to regions to allow you to easily identify or compare subpopulations of
events that appear in different analysis graphs. For more information, see
“Changing Region Color” on page 4-32.
You can make changes to the existing scattergrams or add more scattergrams.
➣ To create a new scattergram, select View ⇒ New Scattergram from the menu bar or
right-click on the Workspace and select New Scattergram.
Note: Scattergram and Histogram windows are numbered consecutively.
Scattergrams have an S prefix, histograms have an H prefix.
A new scattergram is added to your Workspace.
4-22 iCys Research Imaging Cytometer User Guide Revision B