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Glossary Glossary of Terms<br />
encoded in it, allowing more shades to be recorded and shown. 8 bits can<br />
reproduce a photo-realistic 256 shades of gray.<br />
group box A standard control used to group a set of options in a dialog box.<br />
H<br />
hard disk A permanent storage device either mounted inside a computer or as an external<br />
device in its own case.<br />
HeNe laser (Helium-neon) A light source emitting red light at 633 nm with a power output of<br />
approximately 5 mW. (This definition is specific for the <strong>iCys</strong>.)<br />
histogram A two-dimensional graphical means of presenting data for measured features. The<br />
horizontal axis of the represents signal intensity of the feature while the vertical<br />
axis represents the number of events (counts).<br />
I<br />
icon A symbol on the screen that simplifies access to a program, command, or data file.<br />
<strong>iCys</strong> Cytometric<br />
Analysis<br />
Software<br />
<strong>iCys</strong> Research<br />
Imaging<br />
Cytometer<br />
immunofluorescence<br />
The software application that configures the <strong>iCys</strong> system, governs the scanning<br />
operations, and collects the scan data.<br />
The laser scanning cytometer that includes the scanning mirrors, lenses,<br />
photomultipliers and all other components required to perform the laser scanning<br />
operations.<br />
The use of fluorocarbon-labeled antibodies to detect or quantitate cell surface<br />
markers.<br />
insertion point The position, indicated by a blinking vertical bar, at which text is entered into a<br />
report.<br />
integration<br />
contour<br />
An event contour that is a fixed number of pixels outside the threshold contour.<br />
The event’s total or integrated fluorescence is computed by adding all the pixel<br />
values within the contour.<br />
isometric plot A plot mode in which two user-selected features are displayed with the frequency<br />
of occurrence being represented as a 3D surface.<br />
L<br />
laser (Light Amplification by Simulated Emission of Radiation) A device that converts<br />
various frequencies of light into one small and extremely intense beam of one<br />
wavelength radiation.<br />
laser scan image A bit map image of the raw data that is detected by the <strong>iCys</strong> for a scanned area of<br />
the slide.<br />
lens A transparent refracting device in a microscope, usually made of glass, that<br />
changes the shape of a beam of light.<br />
D-6 <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> Revision B
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