iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
number of X Steps to generate a square scan field. The scan field and pixel
size are determined by the dimensions you select and are displayed in the
Scan field dimension window below.
❍ Scan field dimension—Displays the pixel size and size of the scan field
based on the parameters you have selected.
❍ Stop on count—Checking this box will stop the scan when the number of
events in the designated Region reaches the designated count.
Note that when the count has been reached, the system might scan one extra
field since events are analyzed while scanning the next field. Events from the
extra field will be ignored when stop on count has been reached.
❍ Advanced Tab—Clicking the Advanced tab opens a menu in which you can
specify whether or not to apply pixel correction, the Y interval in microns,
whether or not to save field images, and, if so, in what format. You can also
choose to save mosaic images for a Test Scan, a scan and save, or both.
❍ Scan Mode—Describes current scan (Virtual, Live, Bypass)
❍ Correct Pixels—Checking this option enables scanline correction to account
for variations in collection efficiency along the scanline. This is performed on
each of the images as they are acquired, and is shown in the figure below.
3-50 iCys Research Imaging Cytometer User Guide Revision B