iCys User Guide

More documents

Recommendations

Info

Administrator Tasks Forward Scatter Pixel Correction Forward Scatter Pixel Correction When images are collected using the forward scatter mechanism, variations in pixel intensity are produced because the laser beam strikes the sensor at various angles during its “sweep.” In order to produce the best quality images, these variations must be corrected. The Administration menu offers a Forward Scatter Pixel Correction utility. The opening screen is shown below: The textbox on the left contains instructions on how to proceed. List boxes allow selection of a laser and objective. Each combination of laser and objective must be corrected. Once selections are made, press the Measure button. The utility will move the empty stage to the center and automatically adjust the gain and offset for the best exposure. The textbox on the left will display the steps of the measurement. The minimum and maximum values of pixel brightness required to pass these adjustments are stored in the calibration file under “scatter-auto-exposure.” The factory settings should work in most circumstances. As the adjustments progress, you can see the results in the image window. When the adjustments are complete, the textbox will display the message “Scan complete.” When the measurement is complete, a message box will display the message “Measurement complete.” After clicking OK on the message box, you will note that the Save button has become available. You may elect to save the correction factors now or wait until you make another measurement. When the Measure button is pressed again, the current correction data will be replaced with new data. C-26 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Administrator Tasks Before saving, it is wise to review the results of the correction. This can be done by pressing the Test button with the Correct Pixels checkbox checked. The utility will collect and display one scan field of data, either corrected or uncorrected, depending on whether the checkbox is checked. Once you are satisfied, the data can be saved. If the utility is exited with data unsaved a message will be displayed asking if the data should be saved or not. Revision B iCys Research Imaging Cytometer User Guide C-27
Page 1 and 2:
User Guide DOC190-0037-001 Revision
Page 3 and 4:
Contents Chapter 1 Introduction Man
Page 5 and 6:
May 6, 2005 Contents iCys Status Ba
Page 7 and 8:
May 6, 2005 Contents Appendix B Usi
Page 9 and 10:
Chapter 1 Introduction This manual
Page 11 and 12:
May 6, 2005 Introduction System Ove
Page 13 and 14:
Chapter 2 iCys Research Imaging Cyt
Page 15 and 16:
May 6, 2005 iCys Research Imaging C
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Chapter 3 iCys Cytometric Analysis
Page 49 and 50:
May 6, 2005 iCys Cytometric Analysi
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Chapter 4 Generating Analytical Dat
Page 149 and 150:
May 6, 2005 Generating Analytical D
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Appendix A Installing iCys Research
Page 199 and 200:
May 6, 2005 Installing iCys Researc
Page 201 and 202:
May 6, 2005 Installing iCys Researc
Page 203 and 204:
Appendix B Using the iNovator Toolk
Page 205 and 206:
May 6, 2005 Using the iNovator Tool
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216: May 6, 2005 Using the iNovator Tool
Page 241 and 242: Appendix C Administrator Tasks This
Page 243 and 244: May 6, 2005 Administrator Tasks Dis
Page 245 and 246: May 6, 2005 Administrator Tasks Usi
Page 247 and 248: May 6, 2005 Administrator Tasks Def
Page 249 and 250: May 6, 2005 Administrator Tasks 5.
Page 251 and 252: May 6, 2005 Administrator Tasks The
Page 253 and 254: May 6, 2005 Administrator Tasks Det
Page 255 and 256: May 6, 2005 Administrator Tasks ❍
Page 261 and 262: May 6, 2005 Administrator Tasks ✧
Page 263 and 264: May 6, 2005 Administrator Tasks Det
Page 265: May 6, 2005 Administrator Tasks 5.
Page 269 and 270: Appendix D Glossary This chapter co
Page 271 and 272: May 6, 2005 Glossary C carrier The
Page 273 and 274: May 6, 2005 Glossary EVT file An iC
Page 275 and 276: May 6, 2005 Glossary linear amplifi
Page 277 and 278: May 6, 2005 Glossary region A user-
Page 279 and 280: May 6, 2005 Glossary V voltage The
show all

iCys User Guide

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?