iCys User Guide

Administrator Tasks Defining Custom Carriers
Well section of the XY tab to set the location. The X and Y margin parameters are
automatically filled in by iCys.
Note: Always turn the oscilloscope off and wait for the Current X and Current Y
values (at the bottom of the XY tab) to update before you click Set from
X,Y.
The update may take a few moments, but will ensure that the
measurement is correct.
Setting the Interval using iCys
➣ To set the interval, as described in Step 5, above:
1. Turn on the oscilloscope trace, and open the Argon Laser Shutter, then select B2
from the Well pull-down list in the Other Wells section of the XY tab.
2. View the beam, using a piece of thin white paper placed over well B2, and adjust
the stage so the beam is centered in the well.
3. Turn off the oscilloscope and click the Set from X,Y button in the Other Wells
section. The Interval parameter is automatically filled in by iCys.
4. To make sure that the interval is correct, select the last well H12 for a 96-well
plate) in your microplate from the Other Wells Well pull-down list and click
Move To to drive the stage to that well. Check that the beam is centered on the
last well, again using thin white paper. If it is not centered, adjust the interval (but
do not use the last well) and check again using the last well.
Determining the Microplate Z Parameters
Finding the Base to Well Height
➣ To determine the Base to Well Height:
1. Click on the Z tab in the Edit Carrier window.
2. With the stage at well A1, the same objective in place (as displayed in the task
bar), and the Oscilloscope window open and on, and the Argon Laser Shutter
open, use the rocker switch on the Remote Control unit to drive the objective
upwards slowly until you observe a reasonably well focused line or lines on the
autofocus monitor. Make sure that you are focusing on the first line or pair of lines
to be imaged on the display.
3. Use the Fine Focus knob on the Remote Control unit to create a well focused
image.
4. Click Set from Z for the Base To Well Height field in the Z tab. Mark down this
value, and then take the same measurement at a number of other points on the
plate, such as:
❍ The other three corner wells
❍ A well near the center of the plate
C-14 iCys Research Imaging Cytometer User Guide Revision B