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May 6, 2005 Glossary<br />
linear amplifier An electronic device which produces an output current or voltage directly<br />
proportional to the input current or voltage.<br />
log scale A scale used to increase the dynamic range of the displayed data.<br />
M<br />
Max Pixel One of the measured features of an event. It is the measurement of the pixel within<br />
the event contour having the largest value (highest intensity of fluorescence or<br />
scatter).<br />
mean A value calculated by finding the sum of the channel numbers from all the cells in<br />
the sample and dividing it by the total number of cells in the sample.<br />
median A value obtained by lining up the cells in order of increasing intensity and taking<br />
the channel number of the cell that is at the midpoint in that sequence.<br />
mode See peak<br />
multiplexing The process in computers and instrumentation of switching several different<br />
analog signals in succession to the input of a single analog-todigital<br />
converter and then storing the output data in sequence.<br />
N<br />
network A group of interconnected computers which are linked together for the purpose of<br />
sharing files, programs, or peripherals.<br />
normalize A software process of automatically setting the scale and offset so that:<br />
0<br />
❑ An axis displaying Max Pixel makes a cell with the maximum possible Max<br />
Pixel appear in the last channel<br />
❑ An axis displaying Scan makes a cell with the maximum possible scan<br />
number (767) appear in the last channel<br />
objective A microscope lens which magnifies the specimen on the stage by a specified<br />
power (such as 10X, 20X, or 40X).<br />
offset A shift in the position of the data in a histogram or scattergram relative to its<br />
original position. An offset results in a shift of the data along the selected axis.<br />
operating<br />
system<br />
(OS) The software that controls the execution of computer programs of a specific<br />
computer system.<br />
optical filter An optical component which either transmits or attenuates incident light of<br />
specific wavelength.<br />
optical<br />
sectioning<br />
A series of scans of the same well or scan area at different focal points. The result<br />
is a series of scans that move through the sample from top to bottom by a specified<br />
distance. Optical sectioning is a form of repetitive scanning.<br />
Revision B <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> D-7
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