iCys User Guide

May 6, 2005 iCys Cytometric Analysis Software Application Overview
Uncorrected laser scan image
exhibiting roll-off
❍ Y Interval—Allows you to specify a gap, in microns, to add between scan
field rows.
If the distance = 0, the scans are contiguous.
Scan strips (that is, scan field rows) are 384µ wide. Therefore, if you set the Y
interval to 384, you can perform a fast scan by scanning every other row.
For example:
Y interval = 0
Y interval = 384
Corrected laser scan image
Tissue Scanned with Y = 384
In the example above, you can clearly see the scan strips in the tissue.
❍ Field image—Choose whether or not to save a field image, and in what
format.
✧ JPEG—If Raw files are saved in Jpeg format (.jpg extension), some
minor differences may be seen between these files and the live data,
however, the size of the files is greatly reduced with Jpeg files.
✧ Flat—ImagePro flat files (.flf extension) store the identical pixel values
as are used in the live scan, however, flat files are very storage intensive.
❍ Mosaic image—Choose whether or not to save a mosaic image, or to view a
mosaic image during a Test Scan.
❍ Pixel size—Select the pixel size in microns for the mosaic image. A smaller
pixel size gives better image resolution. A larger pixel size decreases storage
space needed for the image.
❍ Sampling—Determines the property used to create the mosaic image.
✧ Single creates the image based on the first pixel in the sampling bin.
✧ Average creates the image based on the average value of the pixels in the
sampling bin.
✧ MaxPixel creates the image based on the pixel in the sampling bin with
the maximum value.
Revision B iCys Research Imaging Cytometer User Guide 3-51