iCys User Guide

May 6, 2005 iCys Research Imaging Cytometer Hardware Reference
➣ To load a 4-slide carrier:
1. Snap the slides into their respective slide holders on the underside of the carriers,
coverslip down.
Note: Since 4-slide carriers are loaded upside down, they are numbered from
right to left.
2. Ensure that the slides are secure.
Tip: For slide carriers, make sure that the slides themselves are loaded into the
tray with the cover side on the bottom (that is, upside down). Also, make
sure that each slide is loaded into its holder correctly. The slides must be
loaded flush with the mounting surface on the bottom of the carrier.
3. Invoke the load operation; click Load on the taskbar or select Instrument ⇒
Load Plate from the menu bar.
4. Place the slide holder on the stage with the bevel at the lower left. Ensure that the
slide holder is secure.
Note: Do not touch the coverslip. Any contamination can adversely affect your
data.
Loading a 3-Petri Dish Holder
You can order 3-petri dish holders from CompuCyte. These holders allow you to scan
multiple samples with less human intervention. The 3-petri dish holder fits into the
same space on a stage as a microtiter plate.
Note: If your iCys system does not use the optional AutoFocus feature, make
sure that you only scan one petri dish at a time. Between petri dishes in
the same carrier, you must lower the objective turret before moving the
stage.
Revision B iCys Research Imaging Cytometer User Guide 2-21