iCys User Guide

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Generating Analytical Data Contours Green channel cleared Using Inverted Scatter for Contouring Using the Inverted Scatter channel for contouring allows you to generate precise contours on events with little or no fluorescence. When you use the Light Loss scatter position, events appear dark on a bright background (light loss due to absorbance and refraction is measured). Therefore, using Inverted Scatter generates bright events with a dark background. With appropriate settings, the bright events can be contoured. ➣ To use Scatter for contouring: 1. In the Protocol ⇒ Detectors window choose the Virtual Channel tab. 2. Create a virtual channel using Inverse Scatter. Give the channel a meaningful name (such as ScatterInvert). For details on creating a virtual channel, see “Creating Virtual Channels” on page 3-43. 3. In the Scatter tab of the Detectors window, select Light Loss as the Scatter position. 4. In the Protocol ⇒ Detectors ⇒ Virtual Channel tab, check the name, e.g. ScatterInvert, of the new virtual channel. 5. Create a new channel module using the inverse scatter virtual channel. For additional information on channel modules see “Channel” on page 3-52. When you run iCys, the contoured events will appear bright on a dark background, as shown: 4-14 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Generating Analytical Data Inverse Scatter channel Scatter channel Revision B iCys Research Imaging Cytometer User Guide 4-15
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Contents Chapter 1 Introduction Man
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Chapter 1 Introduction This manual
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Chapter 2 iCys Research Imaging Cyt
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